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The Effect of Melatonin on the Secretion of Progesterone in Sheep and on the Development of Ovine Embryos In Vitro

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Abstract

Two experiments were carried out in order to determine whether melatonin can improve secretion of progesterone in vivo, and its effect on embryonic development in vitro. In the first experiment, blood samples were collected from 5 ewes at 15 min intervals for 2 h at 7 and 10 days after withdrawal of progestagen pessaries. The first hour constituted a control period, which ended with an intravenous administration of 3 μg/(kg bw)0.75 melatonin. All the ewes on day 7 and three of the ewes on day 10 showed a progesterone response to melatonin challenge, defined as an increase in the plasma progesterone concentration in at least two consecutive samples during the post-treatment period above the mean+2SD of the values in the pre-treatment period. A paired t-test revealed a significant effect of melatonin on the overall plasma progesterone concentrations before and after the challenge, both on day 7 (pre, 0.61±0.11; post, 0.73±0.13 ng/ml; p<0.01) and day 10 (pre, 1.16±0.19; post, 1.30±0.20 ng/ml; p<0.05). Ninety-one thawed embryos (46 morulae and 45 blastocysts) were used in the second experiment, being cultured with or without 1 μg/ml melatonin. If the embryos were blastocysts when the culture started, melatonin increased the percentage that had hatched after 24 h of culture (p<0.01), and there was a lower percentage of degenerated embryos at the end of the incubation period (p<0.05). It may be concluded that melatonin treatment in sheep can increase both fertility and prolificacy by improving luteal function and embryonic survival.

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REFERENCES

  • Ashworth, C.J., Sales, D.I. and Wilmut, I., 1989. Evidence of an association between the survival of embryos and the periovulatory plasma progesterone concentration in the ewe. Journal of Reproduction and Fertility, 87, 23-32

    Google Scholar 

  • Beard, A.P., Hunter, M.G. and Lamming, G.E., 1994. Quantitative control of oxytocin-induced PGF2α release by progesterone and oestradiol in ewes. Journal of Reproduction and Fertility, 100, 143-150

    Google Scholar 

  • Bittman, E.L., Karsch, F.J. and Hopkins, J.W., 1983. Role of the pineal gland in ovine photoperiodism: regulation of seasonal breeding and negative feedback effects of estradiol upon luteinizing hormone secretion. Endocrinology, 113, 329-336

    Google Scholar 

  • Cagnacci, A., Krauchi, K., Wirzjustice, A. and Volpe, A., 1997. Homeostatic versus circadian effects of melatonin on core body temperature in humans. Journal of Biological Rhythms, 12, 509-517

    Google Scholar 

  • Chemineau, P., Vandaele, E., Brice, G. and Jardon, C. 1991. Utilisation des implants de mélatonine pour l'amélioration des performances de reproduction chez la brebis. Recueil de Médecine Vétérinaire, 167, 227-239

    Google Scholar 

  • Durotoye, L.A., Argo, C.M., McNeil, M.E., Graham, N.B. and Rodway, R.G., 1985. Early lambing and increased lambing percentage with slow-release hydrogel melatonin implants. Journal of Reproduction and Fertility, Abstract Series, 1, 83

    Google Scholar 

  • Durotoye, L.A., Webley, G.E. and Rodway, R.G., 1997. Stimulation of the production of progesterone by the corpus luteum of the ewe by the perfusion of melatonin in vivo and by treatment of granulosa cells with melatonin in vitro. Research in Veterinary Science, 62, 87-91

    Google Scholar 

  • Espinar, A., Osuna, C., Feliu, C. and Guerrero, J.M., 1994. High-activity of retinal N-acetyltransferase in the early development of the chick embryo. Independence of lighting conditions. Neuroscience Letters, 179, 103-106

    Google Scholar 

  • Forcada, F., Zarazaga, L. and Abecia, J.A., 1995. Effect of exogenous melatonin and plane of nutrition after weaning on estrous activity, endocrine status and ovulation rate in Salz ewes lambing in the seasonal anoestrus. Theriogenology, 43, 1179-1193

    Google Scholar 

  • Forcada, F., Abecia, J.A., Lozano, J.M. and Zúñiga, O., 2000. Repeated superovulation of high-prolificacy Rasa Aragonesa ewes before culling as an inexpensive way to obtain high-quality embryos. Livestock Production Science, 66, 263-269

    Google Scholar 

  • Haresign, W., Peters, A.R. and Staples, L.D., 1990. The effect of melatonin implants on breeding activity and litter size in commercial sheep flocks in the UK. Animal Production, 50, 111-121

    Google Scholar 

  • Iuvone, P.M. and Gan, J.W., 1994. Melatonin receptor-mediated inhibition of cyclic-AMP accumulation in chick retinal cell-cultures. Journal of Neurochemistry, 63, 118-124

    Google Scholar 

  • Koumitzis, S.A., Belibasaki, S. and Doney, J.M., 1989. Melatonin advances and condenses the onset of seasonal breeding in Greek dairy ewes. Animal Production, 48, 399-405

    Google Scholar 

  • McElhinny, A.S., Davis, F.C. and Warner, C.M., 1996. The effect of melatonin on cleavage rate of C57BL/6 and CBA/Ca preimplantation embryos cultured in vitro. Journal of Pineal Research, 21, 44-48

    Google Scholar 

  • McEvoy, T.G., Robinson, J.J., Aitken, R.P. and Robertson, I.S., 1998. Melatonin treatments of embryo donor and recipient ewes during anestrus affects their endocrine status, but not ovulation rate, embryo survival or pregnancy. Theriogenology, 49, 943-955

    Google Scholar 

  • McMillan, W.H. and Sealey, R.C., 1989. Do melatonin implants influence the breeding season in Coopworth ewes? Proceedings of the New Zealand Society of Animal Production, 49, 43-46

    Google Scholar 

  • Naitana, S., Dattena, M., Gallus, M., Loi, P., Branca, A., Ledda, S. and Cappai, P., 1995. Recipient synchronization affects viability of vitrified ovine blastocysts. Theriogenology, 43, 1371-1378

    Google Scholar 

  • Naitana, S., Loi, P., Ledda, S., Cappai, P., Dattena, M., Bogliolo, L. and Leoni, G., 1996. Effect of biopsy and vitrification on in vitro survival of ovine embryos at different stages of development. Theriogenology, 46, 813-824

    Google Scholar 

  • Naitana, S., Ledda, S., Loi, P., Leoni, G., Bogliolo, L., Dattena, M. and Cappai, P., 1997. Polyvinyl alcohol as a defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. Animal Reproduction Science, 48, 247-256

    Google Scholar 

  • Rondón, Z., Forcada, F., Zarazaga, L., Abecia, J.A. and Lozano, J.M., 1996. Oestrus activity, ovulation rate and plasma melatonin concentrations in Rasa Aragonesa ewes maintained at two different and constant body condition score levels and implanted or reimplanted with melatonin. Animal Reproduction Science, 41, 225-236

    Google Scholar 

  • Russel, A.J.F., Doney, J.M. and Gunn, R.G., 1969. Subjective assessment of body fat in live sheep. Journal of Agricultural Science, Cambridge, 72, 451-454

    Google Scholar 

  • Ubeda, A., Trillo, M.A., House, D.E. and Blackman, C.F., 1995. Melatonin enhances junctional transfer in normal C3H/10T1/2 cells. Cancer Letters, 91, 241-245

    Google Scholar 

  • Wallace, J.M., Robinson, J.J., Wigzell, S. and Aitken, R.P., 1988. Effects of melatonin on the peripheral concentrations of LH and progesterone after oestrus, and on conception rate in ewes. Journal of Endocrinology, 119, 523-530

    Google Scholar 

  • Webley, G.E. and Luck, M.R., 1986. Melatonin directly stimulates the secretion of progesterone by human and bovine granulosa cells in vitro. Journal of Reproduction and Fertility, 78, 711-717

    Google Scholar 

  • Webley, G.E., Luck, M.R. and Hearn, J.P., 1988. Stimulation of progesterone secretion by cultured human granulosa cells with melatonin and catecholamines. Journal of Reproduction and Fertility, 84, 669-677

    Google Scholar 

  • Winterberger-Torres, S. and Sevellec, C., 1987. Atlas of the Early Development of the Sheep Embryo, (INRA, Paris)

    Google Scholar 

  • Yeates, N.T.M., 1949. The breeding season of the sheep with particular reference to its modification by artificial means using light. Journal of Agricultural Science, 39, 1-43

    Google Scholar 

  • Yie, S.M., Niles, L.P. and Younglai, E.V., 1995. Melatonin receptors on human granulosa cell membranes. Journal of Clinical Endocrinology and Metabolism, 80, 1747-1749

    Google Scholar 

  • Zarazaga, L., Malpaux, B., Guillaume, D., Bodin, L. and Chemineau, P., 1998. Genetic variability in melatonin concentrations in ewes originates in its synthesis not in its catabolism. American Journal of Physiology, 274, 1086-1090

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Miguel Servet 177, 50013, Zaragoza, Spain

    J.A. Abecia, F. Forcada & O. Zúñiga

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  1. J.A. Abecia
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  2. F. Forcada
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  3. O. Zúñiga
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Abecia, J., Forcada, F. & Zúñiga, O. The Effect of Melatonin on the Secretion of Progesterone in Sheep and on the Development of Ovine Embryos In Vitro . Vet Res Commun 26, 151–158 (2002). https://doi.org/10.1023/A:1014099719034

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