Skip to main content
SpringerLink
Log in

RFLP analysis of genetic variation in species of section Arachis, genus Arachis (Leguminosae)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Four A-genome species of the genus Arachis (A. cardenasii, A. correntina, A. duranensis, A. kempff-mercadoi), three B genomes species (A. batizocoi, A. ipaënsis and A. magna),the AABB allotetraploid A. hypogaea (cultivated peanut) and introgression lines resulting from a cross between A. hypogaea and A. cardenasii were analyzed by RFLP. The A genome species (cytologically characterized by the presence of a small chromosome pair ‘A’) were closely similar to each other and shared a large number of restriction fragments. In contrast, the B genome species differed more from one another and shared few fragments. The results of this study indicate that the absence of the small chromosome pair is not a good criterion for grouping species of section Arachis as B genome species, since their genome might be quite distinct from the B genome of A. hypogaea.The lowest genetic variation was detected within accessions of A. duranensis (17 accessions), followed by A. batizocoi (4 accessions) and A. cardenasii (9 plants of accession GKP 10017).The high level of genetic variation found in A. cardenasii might indicate that not all accessions of wild species of Arachis are autogamous, as reported for A. hypogaea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bianchi-Hall, C.M., R.D. Keys., H.T. Stalker & J.P. Murphy, 1993. Diversity of seed storage protein pattern in wild peanut (Arachis, Fabaceae) species. Pl Syst Evol 186: 1–15.

    Article  CAS  Google Scholar 

  • Cerry, T.A., G. Caetano-Anollés, R.N. Trigiano & T.W. Starman, 1996. Molecular phylogeny and DNA amplification fingerprinting of Petunia taxa. Theor Appl Genet 92: 1009–1016.

    Article  Google Scholar 

  • Curtis, M.D., D.F. Cameron & J.M. Manners, 1995. Molecular evidence that diploid Stylosanthes humilis and diploid Stylosanthes hamata are progenitors of allotetraploid Stylosanthes hamata cv. Verano. Genome 38: 344–348.

    CAS  PubMed  Google Scholar 

  • Dias, J.S., M.B. Luna, K.M. Song, A.A. Monteiro, P.H. Williams & T.C. Osborn, 1992. Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs. Euphytica 58: 221–229.

    Article  Google Scholar 

  • Diers, B.W. & T.C. Osborn, 1994. Genetic diversity of oilseed Brassica napus germplasm based on restriction fragment length polymorphisms. Theor Appl Genet 88: 662–668.

    Article  Google Scholar 

  • Fatokun, C.A., D. Danesh, N.D. Young & E.L. Stewart, 1993. Molecular taxonomy relationships in the genus Vigna based on RFLP analysis. Theor Appl Genet 86: 97–104.

    Article  CAS  Google Scholar 

  • Fernandez, A. & A. Krapovickas, 1994. Cromosomas y evolución en Arachis (Leguminosae). Bonplandia 8: 187–220.

    Google Scholar 

  • Friar, E. & G. Kochert, 1991. Bamboo germplasm screening with nuclear restriction fragment length polymorphism. Theor Appl Genet 82: 697–703.

    Article  CAS  Google Scholar 

  • Friar, E. & G. Kochert, 1994. A study of genetic variation and evolution of Phyllostachys (Bamboosoidae: Poaceae) using nuclear restriction fragment length polymorphisms. Theor Appl Genet 89: 265–270.

    Article  Google Scholar 

  • Futuyma, D.J., 1992. Biologia Evolutiva. Sociedade Brasileira de Genética / CNPq, Ribeirão Preto, São Paulo, Brazil.

    Google Scholar 

  • Garcia, G.M., H.T. Stalker & G. Kochert, 1995. Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers. Genome 38: 166–176.

    PubMed  CAS  Google Scholar 

  • Galgaro, L., C.R. Lopes, M. Gimenes, J.F.M. Valls & G. Kochert, 1998. Genetic variation between several species of section Extranervosae, Caulorrhizae, Heteranthae and Triseminatae (genus Arachis) estimated by DNA polymorphism. Genome 41: 445–454.

    Article  CAS  Google Scholar 

  • Halward, T.M., H.T. Stalker, E.A. Larue & G. Kochert, 1991. Genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and wild species. Genome 34: 1013–1020.

    CAS  Google Scholar 

  • Halward, T.M., H.T. Stalker, E.A. Larue & G. Kochert, 1992. Use of single primer DNA amplification in genetic studies of peanut. Plant Mol Biology 18: 315–325.

    Article  CAS  Google Scholar 

  • Halward, T., H.T. Stalker & G. Kochert, 1993. Development of an RFLP linkage map in diploid peanut species. Theor Appl Genet 87: 379–384.

    Article  CAS  Google Scholar 

  • Hammons, R.O., 1994. The origin and history of the groundnut. In: The Groundnut Crop: A Scientific Basis for Improvement. Chapman and Hall.

  • Kochert, G., T. Halward, W.D. Branch & C.E. Simpson, 1991. RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theor Appl Genet 81: 565–570.

    Article  CAS  Google Scholar 

  • Kochert, G., H.T., Stalker, M. Gimenes, L. Galgaro, C.R. Lopes & K. Moore, 1996. RFLP and cytogenetic evidence on the origin and evolution of allotetraploid domesticated peanut Arachis hypogaea (Leguminosae). Amer J Bot 83: 1282–1291.

    Article  CAS  Google Scholar 

  • Krapovickas, A. & W.C. Gregory, 1994. Taxonomia del genero Arachis (Leguminosae). Bonplandia 8: 1–186.

    Google Scholar 

  • Lanham, P.G., B.P. Forster, P. Mcnicol, J.P. Moss & W. Powell, 1994. Seed storage protein variation in Arachis species. Genome 37: 487–496.

    CAS  PubMed  Google Scholar 

  • Lavia, G.I., 1996. Estudios cromosómicos en Arachis (Leguminosae). Bonplandia 9: 111–120.

    Google Scholar 

  • Link, W., C. Dixkens, M. Singh, M. Schwall & A.E. Melchinger, 1995. Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD markers. Theor Appl Genet 90: 27–32.

    Article  CAS  Google Scholar 

  • Lu, J. & B. Pickersgill, 1993. Isozyme variation and species relationships in peanut and its wild relatives (Arachis L. - Leguminosae). Theor Appl Genet 85: 550–560.

    Article  CAS  Google Scholar 

  • Lu, J., M.R. Knox, M.J. Ambrose, J.K.M. Brown & T.H.N. Ellis, 1996. Comparative analysis of genetic diversity in pea assesses by RFLP and PCR-based methods. Theor Appl Genet 93: 1103–1111.

    Article  CAS  Google Scholar 

  • Miller, J.C. & S.D. Tanksley, 1990. RFLP analysis of phylogenetic relationship and genetic variation in the genus Lycopersicon. Theor Appl Genet 80: 437–448.

    CAS  Google Scholar 

  • Mori, N., Y.G. Liu & K. Tsunewaki, 1995. Wheat phylogeny determined by RFLP analysis of nuclear DNA. 2. Wild tetraploid wheats. Theor Appl Genet 90: 129–134.

    Article  CAS  Google Scholar 

  • Paik-Ro, O.G., R.L. Smith & D.A. Knaulf, 1992. Restriction fragment length polymorphism evaluation of six peanut species within the Arachis section. Theor Appl Genet 84: 201–208.

    Article  CAS  Google Scholar 

  • Van de Peer, Y., R. De Wachter, 1994. Treecon for windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Applic Biosci 10: 569–570.

    CAS  Google Scholar 

  • Petersen, L., H. Østergård & H. Giese, 1994. Genetic diversity among wild and cultivated barley as revealed by RFLP. Theor Appl Genet 89: 676–681.

    Article  CAS  Google Scholar 

  • Singh, A.K. & J.P. Moss, 1982. Utilization of wild relatives in genetic improvement of Arachis hypogaea L. Part 2: Chromosome complement of species in section Arachis. Theor Appl Genet 61: 305–314.

    Google Scholar 

  • Singh, A.K & J.P. Moss, 1984. Utilization of wild relatives in the genetic improvement of Arachis hypogaea L. 5. Genome analysis in section Arachis and its implications in gene transfer. Theor Appl Genet 68: 355–364.

    Article  Google Scholar 

  • Singh, A.K., H.T. Stalker & J.P. Moss, 1990. Cytogenetics and uses of alien genetic variation in groundnut improvement. In: Chromosome Engineering in Plants: Genetics, Breeding, Evolution, Part B, pp. 65–76.

  • Singh, A.K., S. Gurtu & R. Jambunathan, 1994. Phylogenetic relationship in the genus Arachis based on seed protein profiles. Euphytica 74: 219–225.

    Article  Google Scholar 

  • Singh, A.K. & J. Smartt, 1998. The genome donor of the groundnut/ peanut (Arachis hypogaea L.) revisited. Genet Res Crop Evol 45: 113–118.

    Article  Google Scholar 

  • Smartt, J. & W.C. Gregory, 1967. Interspecific cross-compatibility between the cultivated peanut Arachis hypogaea L. and members of the genus Arachis. Oléagineux 22: 455–459.

    Google Scholar 

  • Southern, E.M., 1975. Detection of specific enzyme sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517.

    PubMed  CAS  Google Scholar 

  • Stalker, H.T., 1990. A morphological appraisal of wild species in section Arachis of peanuts. Peanut Science 17: 117–122.

    Article  Google Scholar 

  • Stalker, H.T., 1991. A new species in section Arachis of peanuts with a D genome. Amer J Bot 78: 630–637.

    Article  Google Scholar 

  • Stalker, H.T., J.S. Dhesi & D.C. Parry, 1991. An analysis of B genome species Arachis batizocoi (Fabaceae). Pl Syst Evol 174: 159–169.

    Article  Google Scholar 

  • Stalker, H.T., T.D. Phillips, J.P. Murphy & T.M. Jones, 1994. Variation of isozyme patterns among Arachis species. Theor Appl Genet 87: 746–755.

    Article  CAS  Google Scholar 

  • Stalker, H.T., J.S. Dhesi & G. Kochert, 1995. Variation within the species Arachis duranensis Krap and W.C. Gregory, a possible progenitor of cultivated peanut. Genome 38: 1201–1212.

    CAS  PubMed  Google Scholar 

  • Subrahmanyam, P., A.M. Ghanekar, B.L. Nolt, D.V.R. Reddy & D. Mc Donald, 1985. Resistance to groundnut diseases in wild Arachis species. In: Proc Int Workshop on Cytogenetics of Arachis,ICRISAT., India, Patancheru, pp. 49–55.

    Google Scholar 

  • Valls, J.F.M. & E.A. Pizarro, 1994. Collection of wild Arachis germplasm. In: P.C. Kerridge & B. Hardy (Eds.), Biology and Agronomy of Forage Arachis. Cali, CIAT, chapter 1, p. 1–18.

    Google Scholar 

  • Van de Peer, Y. & De Wachter, R., 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Biosci. 30: 569–570.

    Google Scholar 

  • Xu, W.W. & D.A. Sleeper, 1994. Phylogeny of tall fescue and related species using RFLPs. Theor Appl Genet 88: 685–690.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Genética, Universidade Estadual Paulista (UNESP), Rubião Junior, Botucatu, 18618, São Paulo, Brazil

    Marcos A. Gimenes, Catalina R. Lopes & Maria L. Galgaro

  2. (CENARGEN), SAIN, Pq. Rural., Embrapa Recursos Genéticos e Biotecnologia, C.P. 02372, CEP, 70849-970, Brasília, DF, Brazil

    Jose F.M. Valls

  3. Department of Botany, University of Georgia, Athens, GA, U.S.A

    Gary Kochert

Authors
  1. Marcos A. Gimenes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Catalina R. Lopes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maria L. Galgaro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jose F.M. Valls
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gary Kochert
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gimenes, M.A., Lopes, C.R., Galgaro, M.L. et al. RFLP analysis of genetic variation in species of section Arachis, genus Arachis (Leguminosae). Euphytica 123, 421–429 (2002). https://doi.org/10.1023/A:1015033700110

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015033700110

Search

Navigation