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FISH mapping of seven cDNA sequences in the pig

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Abstract

Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28–q29, 7q2.6 and 5p11 respectively. The available human—pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL>1w0,4,14, 5q2.4 and 14q22–q23 respectively. The remaining clone representing an EST mapped to 1p24–p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.

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Authors and Affiliations

  1. Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, 750 07, Uppsala, Sweden

    R. Chaudhary & B. P. Chowdhary

  2. Division of Animal Genetics, Department of Animal Science and Animal Health, The Royal Veterinary and Agricultural University, Bulowsvej 13, 1870, Frederiksberg C, Denmark

    A-K. Wintero & M. Fredholm

Authors
  1. R. Chaudhary
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  2. A-K. Wintero
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  3. M. Fredholm
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  4. B. P. Chowdhary
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Chaudhary, R., Wintero, AK., Fredholm, M. et al. FISH mapping of seven cDNA sequences in the pig. Chromosome Res 5, 545–549 (1997). https://doi.org/10.1023/A:1018445820059

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