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Method of Measurement of Pancreatic Elastase II Activity and Postnatal Development of Proteases in Human Duodenal Juice and Bovine and Porcine Pancreatic Tissue

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Abstract

A specific method for pancreatic elastase IIactivity analysis was developed. True elastase IIactivity could be discriminated from that of elastase Iand chymotrypsin. The postnatal development of four pancreatic proteases in the duodenal juice ofchildren and in the pancreatic homogenates of calves andpiglets was measured. The study was carried out onpatients without (14 children) and with (5 children) pancreatic insufficiency. Calves and pigletswere either milk-fed or weaned until slaughter atdifferent ages. Profiles of enzyme development wereglobally similar in milk-fed piglets and calves, while in children without pancreatic insufficiency,no significant change was observed between 4 and 168months. In children with pancreatic insufficiency,enzyme activity was low. In animals, elastase II and chymotrypsin activities were maximal at birth,decreased with age, and probably were associated withthe digestion of milk protein. In contrast, elastase Iand trypsin activities increased markedly after weaning in connection with the intake of solidfood.

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Authors
  1. M. Gestin
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  2. I. Le Huerou-Luron
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  3. J. Peiniau
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  4. E. Thioulouse
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  5. C. Desbois
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  6. G. Le Drean
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  7. D. Feldman
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  8. A. Aumaitre
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  9. P. Guilloteau
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Gestin, M., Huerou-Luron, I.L., Peiniau, J. et al. Method of Measurement of Pancreatic Elastase II Activity and Postnatal Development of Proteases in Human Duodenal Juice and Bovine and Porcine Pancreatic Tissue. Dig Dis Sci 42, 1302–1311 (1997). https://doi.org/10.1023/A:1018826729004

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