Abstract
Increasing the oleic to linoleic acid ratio (O/L) in peanut has positiveeffects on peanut quality and its nutritional value. Δ12-Fattyacid desaturases (Δ12-Fad) have been targeted as logicalcandidates controlling the high oleate trait. A previous study using genomicDNA identified an insertion and a polymorphism resulting in an amino acid changeassociated with the high oleate trait in Spanish-type peanut cultivars. Theobjectives of this research were to use RT-PCR to confirm that the SingleNucleotide Polymorphims (SNPs) identified by analysis of genomic DNA wereexpressed, and to determine if expression patterns for Δ12-Fadwere the same in both seeds and leaves. A polymorphic region of theΔ12-Fad containing a series of nucleotide changes wasamplified, cloned, and sequenced from mRNA of 155 clones of two parental linesand their independent derived backcross lines (IDBLs). The latter differed intheir oleic to linoleic ratio. Data indicated that the “A”insertion and the amino acid change were expressed in both leaf and seed tissue of thehigh and low-intermediate O/L genotypes. It is postulated that several copiesof the Δ12-Fad are present in the genome. It is reasonable toconclude that total activity, and ultimately the O/L ratio, is dependent on thenumber of functional copies. The results provide the basis for an assay toscreen for the high O/L ratio at the molecular level. We also report thepresence of another isozyme of Δ12-Fad with high homology tosoybean isozyme 2 that was expressed in seeds.
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López, Y., Nadaf, H., Smith, O. et al. Expressed variants of Δ12-fatty acid desaturase for the high oleate trait in spanish market-type peanut lines. Molecular Breeding 9, 183–192 (2002). https://doi.org/10.1023/A:1019767825486
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DOI: https://doi.org/10.1023/A:1019767825486