Abstract
In vitro assays demonstrated that toluene-grown cells of Geobacter metallireducens catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formationwas ca. 45% of the specific in vivo rate of toluene consumption. In addition, bssA and bssB, which code for the α and β subunits of benzylsuccinate synthase (BSS), respectively, were found to have sequences in G. etallireducens similar to the only sequences heretofore available (for three denitrifying strains). This is the first report of the presence of BSS in a ferriciron-reducing bacterium; BSS activity has previously been reported in denitrifying, sulfate-reducing, and anoxygenic phototrophic toluene degraders, as well as in a highly enriched methanogenic, toluene-degrading culture.
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Kane, S.R., Beller, H.R., Legler, T.C. et al. Biochemical and genetic evidence of benzylsuccinate synthase intoluene-degrading, ferric iron-reducing Geobacter metallireducens . Biodegradation 13, 149–154 (2002). https://doi.org/10.1023/A:1020454831407
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DOI: https://doi.org/10.1023/A:1020454831407