Abstract
The present study is an attempt to relate environmental factors ofambient water to the physiology of the cichlid, Oreochromisniloticus. Fish were collected at 3 stations along Lake Maryûtand a fourth one in an authorized hatchery as reference. Water analysis showedthat many attributes of the water quality, in particular in the main basin,werefar beyond admissible levels. In response, functional damage of the heart andliver in polluted fish was pointed out in view of the elevated serum enzymes(alanine aminotransferase, lactate dehydrogenase, creatine kinase, alkalinephosphatase and cholinesterase). Alkaline phosphatase seemed to potentiallyindicate excess phosphate in ambient water, whereas, aspartate aminotransferasewas suppressed in fish caught from waters with the highest metalconcentrations.Serologic data of carbohydrate, lipid and protein metabolites drew attention tothe adverse effects on liver, heart and kidney functions of industrial andmunicipal discharges into the fishes habitats.
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References
Adams S.M. and McLean R.B. 1985. Estimation of largemouth bass, Micropterus salmoides Lacepede, growth using the liver somatic index and physiological variables. J. Fish. Biol. 26: 111-126.
Adams S.M., Crumby W.D., Greeley M.S., Rayon M.G. and Schilling E.M. 1992. Relationship between physiological and fish population responses in a contaminant stream. Environmental Toxicology and Chemistry 11: 1549-1557.
Adham K.G., Hassan I.F., Taha N. and Amin T.H. 1999. Impact of Hazardous exposure to metals in the Nile and Delta lakes on the catfish, Clarias lazera. Environ. Monitor. Assess. 54: 107-124.
Adham K.G., Khairalla A., Abu-Shabana M., Abdel-Maguid N. and Abd El-Moneim A. 1997. Environmental stress in Lake Maryût and physiological response of Tilapia zilli. Gerv. J. Environ. Sci. Health A32: 2585-2598.
Almeida-Val V.M., Farias I.P., Silva M.N., Duncan W.P. and Val A.L. 1995. Biochemical adjustments to hypoxia by Amazon cichlids. Braz. J. Med. Biol. Res. 28: 1257-1263.
Andersen D.E., Reid S.D., Moon T.W. and Perry S.F. 1991. Metabolic effects associated with chronically elevated cortisol in rainbow trout (Oncorhynchus mykiss). Can. J. Fish. Aquat. Sci. 48: 1811-1817.
APHA (American Public Health Association) 1992. Standard Methods of Water and Wastewater. 18th edn. American Water Works Association, Water Environment Federation publication. APHA, Washington, D.C., USA.
Barham D. and Trinder P. 1972. An improved color reagent for the determination of blood glucose by the oxadase system. Analyst. 97: 142-145.
Barton B.A. and Iwama G.K. 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu. Rev. Fish. Dis. 1: 3-26.
Belfield A. and Goldberg D. 1971. Enzyme clasification. Enzyme 12: 561-566.
Black E.C., Robertson A.C. and Parker R.R. 1961. Some aspects of carbohydrate metabolism in fish. In: Martin A.W. (ed.), Comparative Physiology of Carbohydrate Metabolism in Heterothermic Animals. Univ. Washington. Press, Seattle, Wash., pp. 89-124.
Bouck G.R. 1966. Changes in Blood and Muscle Composition of Rock Bass (Ambloplites rupestris) as Physiological Criteria of Stressful Conditions. PhD Dissertation, Michigan state university, East Lansing, USA.
Bouck G.R., Schneider P.W., Jacobson J. and Ball R.C. 1975. Characterization and Subcellular localization of leucine aminonapthylamidase (LAN) in rainbow trout (Salmo gairdneri). J. Fish. Res. Bd. Can. 32: 1289-1295.
Casillas E., Meyers M. and Ames W. 1983. Relationship of serum chemistry values to liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride. Aquat. Toxicol. 3: 61-78.
Casillas E. and Ames W. 1986. Hepatotoxic effects of CCl4 on English sole (Parophrys vetulus): Possible indicators of liver dysfunction. Comp. Biochem. Physiol. 48C: 397-400.
Cawson R.A., Mc Carcken A.W. and Marcus P.B. 1982. Pathologic Mechanisms and Human Disease. The C.V. Mosby Company. ST. Louis Toronto., London.
Dampure C.J. 1984. Lactate dehydrognase and cell injury. Cell Biochem. Funct. 2: 144-148.
Ellsaesser C.F. and Clem L.W. 1987. Blood serum chemistry measurements of normal and acutely stressed channel catfish. Comp. Biochem. Physiol. A. 88: 589-594.
El-Rayis O.A. and El-Sabrouti M.A. 1998. Pollution problems and Proposals for Restoration. J. of Arab. Academy for Science & Technology 23: 16-28.
Emmerson B.T. 1973. Chronic lead nephropathy. Kifney Int. 4: 1-5.
Fawcett J.K. and Scott J.E. 1960. A rapid and precise methods for the determination of urea. J. Clin. Pathol. 13: 156-159.
Folmar L.C., Bonomelli S., Moody T. and Gibson J. 1993. The effect of short-term exposure to three chemicals on the blood chemistry of the pinfish (Lagodon rhomboides). Arch. Environ. Contam. Toxicol. 24: 83-86.
Fossati P. and Prencipe L. 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem. 28: 2077-2080.
Fruton J.S. and Simmonds S. 1958. General Biochemistry. 2nd edn. John Wiley and Sons., N.Y., USA, 1077 pp.
Grant B.F. and Mehrle P.M. 1973. Endrin toxicosis in rainbow trout. Journal of the fisheries Research Board of Canada 30: 31-40.
Heath A.G. and Pritchard A.W. 1965. Effects of severe hypoxia on carbohydrate energy stores and metabolism in two species of fresh water. Fish. Physiol. Zool. 38: 325-334.
Hochachka P.W. and Somero G.N. 1973. Strategies of Biochemical Adaptation. W.B. Saunders Co., Philadelphia, 358 pp.
Kaplan A., Ozabo L.L. and Ophem K.E. 1988. Clinical Chemistry: Interpretation and Techniques. 3rd edn. Lea & Febiger, Philadelphia.
Karmen A., Wróblewski F. and LaDue J. 1955. Transaminase activity in human blood [Appendix: Karmen A. A note on the spectrophotometric assay of glutamic-oxalacetic transaminase in human blood serum]. J Clin Invest. 34: 126-133.
Kind P.R.N. and King E.J. 1954. Estimation of plasma phosphatase by determination of hydrolysed phenol with antipyrine. J Clin. Pathol. 7: 322-30.
Krajnović-Ozretić M. 1991. Serum enzymes in fish as biochemical indicators of marine pollution. MAP Technical Reports series No. 48. UNEP, Athens, 1-11.
Krajnović-Ozretić M. and Krajnović-Ozretić B. 1992. Detection and evaluation of hepatic intoxication in fish. In: Gabrielides G.P. (ed.), Workshop on the biological effects of pollutants on marine organisms, Malta, 10-14 Sept 1991. Proceedings of the FAO/UNEP/IOC, MAP Technical Reports, series No. 69., pp. 165-175.
Lee R.M., Gerking S.D. and Jezierska B. 1983. Electrolyte balance and energy mobilization in acid stressed rainbow trout, Salmo gairdneri, and their relation to reproductive stress. Environ. Biol. Fish. 8: 115-123.
Mehrle P.M. and Mayer F.L. 1980. Clinical tests in aquatic toxicology: State of the Art. Environ. Health prospect 34: 139-143.
Moss D.W., Henderson A.R. and Kachmar J.F. 1986. Text Book of Clinical Chemistry. WB.
Neff J.M. 1985. Use of Biochemical Measurements to Detect Pollutant-Mediated Damage to Fish. In: Cardwell R.D., Purdy R. and Bahner R.C. (eds), Aquatic Toxicology and Hazard Assessment: Seventh symposium, ASTM STP 854. American Society for Testing and Materials, Philadelphia, pp. 155-183.
Oikari A. and Soivio A. 1977. Physiological condition of fish exposed to water containing pulp and paper industry wastes and sewage. In: Alabaster J.S. (ed.), Biological Monitoring of Inland Fisheries. Appl. Sci. Publ. Ltd., London, pp. 89-96.
Perrier H., Perrier C., Gudefin Y. and Gras J. 1972. Adrenaline-induced hypercholesterolemia in the rainbow trout (Salmo gairdneri Richardson): A separate study in male and female trout and the effect of adrenergic blocking reagents. Comp. Biochem. Physiol. 43A: 341-347.
Perrier C., Perrier H. and Gras J. 1973. Etude de l'éffet hyperlipémiant de l'adrénaline chez la triute arc-enciel d'élevage (Salmo gairdneri Richardson). Experientia 29: 24.
Pickering A.D. and Pottinger T.G. 1995. Biochemical effects of stress. In: Hochachka P.W. and Mommsen T.P. (eds), Environmental and Ecological Biochemistry. Elsevier, Amsterdam, pp. 349-379.
Raccicot J.G., Gaudet M. and Leray C. 1975. Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: study of CCl4 toxicity and a case of Aeromonas infection. J. Fish. Biol. 7: 825-835.
Reitman S. and Frankel S. 1957. J. Clin. Path. 28: 56.
Richmond W. 1973. Clin. Chem. 19: 1350-1356.
Riley J.P. and Taylor D. 1968. Chelating resin for concentration of the trace elements from sea water and their analytical in conjunction with atomic absorption spectrometry. Anal. Chim. Acta. 40: 479-485.
Ruzak-Skocir B. and Trbojevic-Cepe M. 1990. Study of serum and Cerebrospinal Fluid enzymes in diagnosis and differential diagnosis of cerebrovascular diseases. Neuroolgija 39: 239-250.
Shulman G.E. 1974. Life Cycles of Fish. Physiology and Biochemistry. John Wiley and Sons, New York, N.Y.
Snedecor G.W. and Cochran W.G. 1969. Statistical Methods. 6th edn. The Iowa State University Press, Ames., Iowa, USA.
Sunyer J.O., Gomez E., Navarro V., Quesada J. and Tort L. 1995. Physiological responses and depression of humoral components of the immune system in gilthead sea bream (Sparus aurata) following daily acute stress. Can. J. Fish. Aquat. Sci. 52: 2339-2346.
Swann L. 2000. A Fish Farmer's Guide to Understanding Water Quality. Purdue University, Electronic version: http://ag.ansc.purdue.edu/aquanic/publicat/state/il-in/as-503.htm IIlinois-Indiana Sea Grant Program.
Szasz G., Gruber W. and Bernt E. 1976. Creatine kinase in serum: 1. determination of optimum reaction conditions. Clin. Chem. 22: 650-656.
Thomas L. 1992. Labor und Diagnose. 4th edn. Medizinische Verlagsgesellschaft, Marburg.
Trinder P. 1969. Ann. Clin. Biochem. 6: 24.
Turner L.C. 1970. The Normal Probability Distribution Modern Applied Mathematics. The English. Universities Press, London, pp. 202-207.
USEPA (US Environmental protection Agency) 1987. Quality Criteria for water: 1986. Rep. No. USEPA 440/5-86-001. US Environmental Protection Agency, Washington, DC, USA.
Van Raaij M.T.M., G.E.E.J.M., Van Den Thillart M., Halleneesch B.P.H.M. and Steffens A.B. 1995. Effect of arterially infused catecholamines and insulin on plasma glucose and free fatty acids in carp. Am. J. Physiol 268: R1163-R1170.
Van Vuren J.H., Vander Merwe M. and du Preez H.H. 1994. The effect of copper on the blood chemistry of Clarias gariepinus (Clariidae). Ecotoxicol. Environ. Saf. 29: 187-199.
Weber H. 1966. Quick and simple ultramicromethod for the determination of serum cholinesterase. Dtsch. Med. Wschr. 91: 1927-1932.
Wieser W. and Hinterleitner S. 1980. Serum enzymes in rainbow trout as tools in the diagnosis of water quality. Bull. Environ. Contam. Toxicol. 25: 188-193.
Wilkie M.P., Simmons H.E. and Wood C.M. 1996. Physiological adaptations of rainbow trout to chronically elevated water pH (pH = 9.5). J. Experiment. Zool. 274: 1-14.
William J.P. 1997. Nongenetic variation, genetic environmental interactions and altered gene expression. Temperature, photoperiod, diet, PH and sex-related effects. Comp. Biochem. Physiol. 117A: 11-66.
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Adham, K.G., Ibrahim, H.M., Hamed, S.S. et al. Blood chemistry of the Nile tilapia, Oreochromis niloticus (Linnaeus, 1757) under the impact of water pollution. Aquatic Ecology 36, 549–557 (2002). https://doi.org/10.1023/A:1021137122046
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DOI: https://doi.org/10.1023/A:1021137122046