Abstract
A bacterial artificial chromosome (BAC) library from the dihaploid melon line `PIT92' was constructed with a 6 times coverage of the haploid melon genome. A contig of four BACs around the MRGH63 resistance gene homologue fragment was created. The complete sequence of a 117-kb BAC clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. Separated by a retrotransposon, that contains large long terminal repeats, the second region presents a group of genes with a conserved distribution in two regions of the Arabidopsis genome. The detailed analysis of this region provides a description of the gene structure and the presence of repetitive sequences in a defined fragment of the genome of Cucumis melo.
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van Leeuwen, H., Monfort, A., Zhang, HB. et al. Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana . Plant Mol Biol 51, 703–718 (2003). https://doi.org/10.1023/A:1022573230486
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DOI: https://doi.org/10.1023/A:1022573230486