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Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana

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Abstract

A bacterial artificial chromosome (BAC) library from the dihaploid melon line `PIT92' was constructed with a 6 times coverage of the haploid melon genome. A contig of four BACs around the MRGH63 resistance gene homologue fragment was created. The complete sequence of a 117-kb BAC clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. Separated by a retrotransposon, that contains large long terminal repeats, the second region presents a group of genes with a conserved distribution in two regions of the Arabidopsis genome. The detailed analysis of this region provides a description of the gene structure and the presence of repetitive sequences in a defined fragment of the genome of Cucumis melo.

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Authors and Affiliations

  1. Departament de Genètica Molecular, Institut de Biologia Molecular de Barcelona, CID-CSIC, Jordi Girona 18, 08034, Barcelona, Spain

    Hans van Leeuwen, Amparo Monfort & Pere Puigdomènech

  2. Departament de Genètica Vegetal, IRTA, Ctra. de Cabrils s/n, 08348, Cabrils, Barcelona, Spain

    Amparo Monfort

  3. Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, 2123 TAMU, Texas A&M University, College Station, Texas, 77843-2123, USA

    Hong-Bin Zhang

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  1. Hans van Leeuwen
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  2. Amparo Monfort
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  4. Pere Puigdomènech
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van Leeuwen, H., Monfort, A., Zhang, HB. et al. Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana . Plant Mol Biol 51, 703–718 (2003). https://doi.org/10.1023/A:1022573230486

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