Abstract
In the present study, the nucleotide sequences of the CHS1 gene from dermatophytes and related fungi in the genera Chrysosporium, Epidermophyton, Microsporum and Trichophyton were investigated using molecular methods. About 440-bp genomic DNA fragments of the CHS1 gene from 21 species were amplified by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these fungi showed more than 83% similarity. The molecular taxonomy of the CHS1 gene sequences revealed that Microsporum was genetically distinct from Chrysosporium and Trichophyton, as classified by morphological characteristics.
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Brown A.R., Chen-Wu J.L., Momany M., Young R., Szaniszlo P.J. and Robbins P.W. 1992. Classification of fungal chitin synthases. Proc. Natl. Acad. Sci. USA 89: 519–523.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
Felsenstein J. 1993. PHYLIP (Phylogeny Inference Package), Version 3.5. Department of Genetics, University of Washington, Seattle, WA.
Graser Y., Fari M.el., Vilgalys R., Kuijpers A.F.A., de Hoog G.S., Presber W. et al. 1999. Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region. Med. Mycol. 37: 105–114.
Kano R., Nakamura Y., Watari T., Watanabe S., Takahashi H., Tsujimoto H. et al. 1997. Phylogenetic analysis of 8 dermatophytes species using chitin synthase 1 gene sequences. Mycoses 40: 411–414.
Kano R., Nakamura Y., Watari T., Watanabe S., Takahashi H., Tsujimoto H. et al. 1998. Molecular analysis of chitin synthase 1 (CHS 1) gene sequences of Trichophyton mentagrophytes complex and T. rubrum. Curr. Microbiol. 37: 236–239.
Kano R., Nakamura Y., Watanabe S., Tsujimoto H. and Hasegawa A. 1999. Phylogenetic relation of Epidermophyton floccosum to the species of Microsporum and Trichophyton in chitin synthase 1 (CHS1) gene sequences. Mycopathologia 146: 111–113.
Nino-Vega G.A., Munro C.A., San-blas G., Gooday G.W. and Gow N.A.R. 1999. Differential expression of chitin synthase gene during temperature-induced dimorphic transitions in Paracoccidioides brasiliensis. Med. Mycol. 38: 31–39.
Page R.D.M. 1996. TREEVIEW: An application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12: 357–358.
Chua S.S., Momany M., Mendoza L. and Szanszlo P.J. 1994. Identification of three chitin synthase genes in the dimorphic fungal pathogen Sporothrix schenckii. Curr. Microbiol. 29: 151–156.
Thompson J.D., Higgins D.G. and Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acid Res. 22: 4673–4680.
Weitzman I., McGinnis M.R., Padhye A.A. and Ajello L. 1986. The genus Arthroderma and its later synonym Nannizzia. Mycotaxon 25: 505–518.
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Hirai, A., Kano, R., Nakamura, Y. et al. Molecular taxonomy of dermatophytes and related fungi by chitin synthase 1 (CHS1) gene sequences. Antonie Van Leeuwenhoek 83, 11–20 (2003). https://doi.org/10.1023/A:1022928811306
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DOI: https://doi.org/10.1023/A:1022928811306