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Immobilization of Pseudomonas delafieldii with magnetic polyvinyl alcohol beads and its application in biodesulfurization

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Abstract

Pseudomonas delafieldii was immobilized in magnetic polyvinyl alcohol (PVA) beads using a hydrophilic magnetic fluid, which was prepared by a co-precipitation method. The beads had distinct super-paramagnetic properties and were compared with immobilized cells in non-magnetic PVA beads. Their desulfurizing activity was increased slightly from 8.7 to 9 mmol sulfur kg−1 (dry cell) h−1. The main advantages was that the magnetic immobilized cells maintain a high desulfurization activity and remain in good shape after 7 times of repeated use, while the non-magnetic immobilized cells could only be used for 5 times. Furthermore, the magnetic immobilized cells could be easily collected or separated magnetically from the biodesulfurization reactor.

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Correspondence to Hui-zhou Liu.

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Shan, Gb., Xing, Jm., Luo, Mf. et al. Immobilization of Pseudomonas delafieldii with magnetic polyvinyl alcohol beads and its application in biodesulfurization. Biotechnology Letters 25, 1977–1981 (2003). https://doi.org/10.1023/B:BILE.0000004388.15751.8c

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  • DOI: https://doi.org/10.1023/B:BILE.0000004388.15751.8c

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