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A complete BAC-based physical map of the Arabidopsis thaliana genome

Nature Genetics volume 22, pages 271–275 (1999)Cite this article

Abstract

Arabidopsis thaliana is a small flowering plant that serves as the major model system in plant molecular genetics1. The efforts of many scientists have produced genetic maps that provide extensive coverage of the genome (http://genome-www.stanford.edu/Arabidopsis/maps.html). Recently, detailed YAC, BAC, P1 and cosmid-based physical maps (that is, representations of genomic regions as sets of overlapping clones of corresponding libraries) have been established that extend over wide genomic areas ranging from several hundreds of kilobases2,3 to entire chromosomes4,5,6,7,8,9. These maps provide an entry to gain deeper insight into the A. thaliana genome structure. A. thaliana has been chosen as the subject of the first large-scale project intended to determine the full genome sequence of a plant10. This sequencing project, together with the increasing interest in map-based gene cloning, has highlighted the requirement for a complete and accurate physical map of this plant species. To supply the scientific community with a high-quality resource, we present here a complete physical map of A. thaliana using essentially the IGF BAC library11. The map consists of 27 contigs that cover the entire genome, except for the presumptive centromeric regions, nucleolar organization regions (NOR) and telomeric areas. This is the first reported map of a complex organism based entirely on BAC clones and it represents the most homogeneous and complete physical map established to date for any plant genome. Furthermore, the analysis performed here serves as a model for an efficient physical mapping procedure using BAC clones that can be applied to other complex genomes.

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Figure 2: BAC-based physical map of the A. thaliana genome.

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Acknowledgements

We thank colleagues who contributed information before publication and L. Willmitzer for support during this work. T.M. was supported through a fellowship by the Max-Planck-Society.

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Authors and Affiliations

  1. Max-Planck-Institut für molekulare Pflanzenphysiologie , Karl-Liebknecht-Strasse 25, 14476, Golm, Germany

    Teresa Mozo, Sabine Fischer, Sebastian Kloska & Thomas Altmann

  2. Whitehead Institute/MIT Center for Genome Research , Cambridge, 02141, Massachusetts, USA

    Ken Dewar

  3. Department of Biology, Plant Science Institute, University of Pennsylvania, Philadelphia, 19104, Pennsylvania, USA

    Pat Dunn & Joseph R. Ecker

  4. Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, 14195, Berlin, Germany

    Hans Lehrach

  5. Washington University School of Medicine, Genome Sequencing Center, St. Louis, 63108, Missouri, USA

    Marco Marra

  6. Cold Spring Harbor Laboratory, Cold Spring Harbor, 11724, New York, USA

    Robert Martienssen

  7. GPC Aktiengesellschaft, Lochhamer Strasse 29, 82152, Martinsried, Germany

    Sebastian Meier-Ewert

Authors
  1. Teresa Mozo
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  2. Ken Dewar
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  3. Pat Dunn
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  4. Joseph R. Ecker
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  5. Sabine Fischer
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  6. Sebastian Kloska
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  7. Hans Lehrach
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  8. Marco Marra
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  9. Robert Martienssen
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  10. Sebastian Meier-Ewert
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  11. Thomas Altmann
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Correspondence to Thomas Altmann.

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Mozo, T., Dewar, K., Dunn, P. et al. A complete BAC-based physical map of the Arabidopsis thaliana genome . Nat Genet 22, 271–275 (1999). https://doi.org/10.1038/10334

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