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Complete in vitro maturation of Plasmodium falciparum gametocytes

Abstract

The form of malaria caused by Plasmodium falciparum is probably the most important infectious disease of man. In tropical Africa alone, where malaria affects almost the entire population, it has been estimated that every year the disease causes the death of 1 million children under 14 yr old1. The mosquito vector of malaria is infected when it ingests mature gametocytes in blood taken from a human carrier of the disease. Development of the parasite continues in the mosquito and culminates with the sporozoite stage, which initiates a new infection when injected by the mosquito into a further human host. Research on P. falciparum sporozoites has been severely limited by a general lack of availability of suitable patients with gametocytes. It was hoped that the recently developed continuous cultivation of P. falciparum2 would solve this, as gametocytes are often produced during cultivation. However, we and others soon found that these gametocytes failed to mature in culture3,4, or at best did so only rarely and unpredictably5–7. We report here that addition of hypoxanthine to the culture medium permits the production of mature, infectious P. falciparum gametocytes on a regular basis.

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References

  1. Maugh, T. H. Science 196, 413–416 (1977).

    Article  ADS  Google Scholar 

  2. Trager, W. & Jensen, J. B. Science 193, 673–675 (1976).

    Article  ADS  CAS  Google Scholar 

  3. Vanderberg, J. P., Weiss, M. M. & Mack, S. R. Bull. Wld Hlth Org. 55, 377–392 (1977).

    CAS  Google Scholar 

  4. Jensen, J. B. J. Protozool. 26, 129–132 (1979).

    Article  CAS  Google Scholar 

  5. Carter, R. & Beach, R. F. Nature 270, 240–241 (1977).

    Article  ADS  CAS  Google Scholar 

  6. Carter, R. & Miller, L. H. Bull. Wld Hlth Org. (Suppl.), 57, 37–52 (1979).

    Google Scholar 

  7. Campbell, C. C., Chin, W., Collins, W. E. & Moss, D. M. Trans. R. Soc. trop. Med. Hyg. 74, 668–669 (1980).

    Article  CAS  Google Scholar 

  8. Vanderberg, J. P. J. Protozool. 21, 527–537 (1974).

    Article  CAS  Google Scholar 

  9. Sherman, I. W. Microbiol. Rev. 43, 453–495 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Weiss, M. M. & Vanderberg, J. P. J. Protozool. 23, 547–551 (1976).

    Article  CAS  Google Scholar 

  11. Jacobs, R. L., Miller, L. H. & Koontz, L. C. J. Parasit. 60, 340–343 (1974).

    Article  CAS  Google Scholar 

  12. Carter, R. & Gwadz, R. W. in Malaria Vol. 3 (ed. Kreier, J. P.) 263–297 (Academic, New York, 1980).

    Book  Google Scholar 

  13. Cochrane, A. H., Nussenzweig, R. S. & Nardin, E. H. in Malaria Vol. 3 (ed. Kreier, J. P.) 163–202 (Academic, New York, 1980).

    Book  Google Scholar 

  14. Clyde, D. F., Most, H., McCarthy, V. & Vanderberg, J. P. Am. J. med. Sci. 266, 169–177 (1973).

    Article  CAS  Google Scholar 

  15. Scheibel, L. W., Ashton, S. H. & Trager, W. Expl Parasit. 47, 410–418 (1979).

    Article  CAS  Google Scholar 

  16. Rutledge, L. C., Ward, R. A. & Gould, D. J. Mosquito News 24, 407–419 (1964).

    Google Scholar 

  17. Vanderberg, J. P. & Gwadz, R. W. in Malaria Vol. 2 (ed. Kreier, J. P.) 153–234 (Academic, New York, 1980).

    Google Scholar 

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Ifediba, T., Vanderberg, J. Complete in vitro maturation of Plasmodium falciparum gametocytes. Nature 294, 364–366 (1981). https://doi.org/10.1038/294364a0

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