Abstract
Although the biochemical mechanism of action of phorbol ester tumour promoters is not fully understood, it is known that phorbol ester binding to the cell surface causes rapid changes in calcium flux1,2 and phospholipid metabolism3–7. A protein kinase activity has recently been described which is dependent on calcium and acidic phospholipids and is further enhanced by diacylglycerol8–10. Previously, we have observed that phorbol ester treatment of EL4 mouse thymoma cells causes a rapid decrease in cytosolic calcium, phospholipid-dependent protein kinase (Ca, PL-PK) activity, which is mediated through the specific phorbol ester cell-surface receptors identified on EL4 cells11. We now show that treatment of parietal yolk sacs (PYS-2) cells with biologically active 12-O-tetradecanoyl phorbol-13-acetate (TPA) provokes a rapid decrease in cytosolic Ca, PL-PK activity that is accompanied by a significant increase in the amount of Ca, PL-PK activity associated with the plasma membrane fraction. These results suggest that the rapid and tight association of Ca, PL-PK activity with the plasma membrane may be an early event in mediating some of the effects of phorbol esters.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gunther, G. J. J. biol. Chem. 256, 12040–12045 (1981).
Verma, A. & Boutwell, R. Biochem. biophys. Res. Commun. 101, 375–383 (1981).
Balman, A. & Hecker, E. Biochim. biophys. Acta 362, 457–468 (1974).
Guy, G. & Murray, A. Cancer Res. 42, 1980–1985 (1982).
Wertz, P. W. & Mueller, G. L. Cancer Res. 38, 2900–2904 (1978).
Grove, R. I. & Schimmel, S. D. Biochem. biophys. Res. Commun. 102, 158–164 (1981).
Ohuchi, K. & Levine, L. J. biol. Chem. 253, 4783–4790 (1978).
Takai, Y. J. biol. Chem. 254, 3692–3695 (1979).
Kishimoto, A., Takai, Y., Mori, T., Kikkawa, U. & Nishizuka, Y. J. biol. Chem. 255, 2273–2276 (1980).
Kuo, J. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7039–7043 (1980).
Kraft, A. S., Anderson, W. B., Cooper, H. L. & Sando, J. J. J. biol. Chem. 257, 13193–13196 (1982).
Kraft, A. S. & Anderson, W. B. J. biol. Chem. (submitted).
Castagna, M. et al. J. biol. Chem. 257, 7847–7851 (1982).
Wise, B. C., Raynor, R. L. & Kuo, J. F. J. biol. Chem. 257, 8481–8488 (1982).
Mori, T., Takai, Y., Minakuchi, R., Yu, B. & Nishizuka, Y. J. biol. Chem. 255, 8378–8380 (1980).
Schatzman, R. C., Wise, B. C. & Kuo, J. F. Biochem. biophys. Res. Commun. 98, 669–676 (1981).
Ushiro, H. & Cohen, S. J. biol. Chem. 255, 8363–8365 (1980).
Cohen, S., Carpenter, G. & King, L. Jr, J. biol. Chem. 255, 4834–4842 (1980).
Nilsen-Hamilton, M. & Hamilton, R. T. Nature 279, 444–446 (1979).
Ek, B., Westermark, B., Wasteson, A. & Heldin, C.-H. Nature 295, 419–420 (1982).
Erickson, R. L., Collett, M. S., Erikson, E. & Purchio, A. F. Proc. natn. Acad. Sci. U.S.A. 76, 6260–6264 (1979).
Smith, A. E., Smith, R., Griffin, B. & Fried, M. Cell 18, 915–924 (1979).
Witte, O. N., Dasgupta, A. & Baltimore, D. Nature 283, 826–831 (1980).
Shih, T. Y., Papageorge, A. G., Stokes, P. E., Weeks, M. O. & Scolnick, E. M. Nature 287, 686–691 (1980).
Hynes, R. O. Cell 21, 601–602 (1980).
Krueger, J. G., Garber, E. A., Goldberg, A. R. & Hanafusa, H. Cell 28, 889–896 (1982).
Cooper, R. A., Braunwald, A. D. & Kuo, A. L. Proc. natn. Acad. Sci. U.S.A. 79, 2865–2869 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kraft, A., Anderson, W. Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membrane. Nature 301, 621–623 (1983). https://doi.org/10.1038/301621a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/301621a0
This article is cited by
-
Unifying principles of bifunctional, proximity-inducing small molecules
Nature Chemical Biology (2020)
-
Rational design and validation of an anti-protein kinase C active-state specific antibody based on conformational changes
Scientific Reports (2016)
-
A fatty acid-dependent hypothalamic–DVC neurocircuitry that regulates hepatic secretion of triglyceride-rich lipoproteins
Nature Communications (2015)
-
Angiotensin(1–7) attenuates the progression of streptozotocin-induced diabetic renal injury better than angiotensin receptor blockade
Kidney International (2015)
-
Protein kinase Cɛ inhibition restores megakaryocytic differentiation of hematopoietic progenitors from primary myelofibrosis patients
Leukemia (2015)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.