Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture

Abstract

The polyoma virus middle-T and the T24 Harvey ras1 genes are individually unable to transform primary baby rat kidney cells. Adenovirus early region 1A provides functions required by these genes to transform primary cells following DNA-mediated gene transfer. These results suggest that separate establishment and transforming functions are required for oncogenic transformation of primary cells in culture.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Tooze, J. (ed.) Molecular Biology of Tumor Viruses Pt 2 (revised) (Cold Spring Harbor Laboratory, New York, 1981).

  2. Houweling, A., Van den Elsen, P. & Van der Eb, A. Virology 105, 537–550 (1980).

    Article  CAS  PubMed  Google Scholar 

  3. Rassoulzadegan, M. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  4. Van der Eb, A. J. et al. Cold Spring Harb. Symp. quant. Biol. 44, 383–399 (1980).

    Article  CAS  PubMed  Google Scholar 

  5. Triesman, R., Novak, U., Favoloro, J. & Kamen, R. Nature 292, 595–600 (1982).

    Article  ADS  Google Scholar 

  6. Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1981).

    Article  ADS  Google Scholar 

  7. Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).

    Article  CAS  PubMed  Google Scholar 

  8. Pulciani, S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2845–2849 (1982).

    Article  ADS  CAS  Google Scholar 

  9. Shimizu, K., Goldfarb, M., Perucho, M. & Wigler, M. Proc. natn. Acad. Sci. U.S.A. 80, 383–387 (1983).

    Article  ADS  CAS  Google Scholar 

  10. Goubin, G., Goldman, D. S., Luce, J., Neiman, P. E. & Cooper, G. M. Nature 302, 114–119 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Rassoulzadegan, M. et al. Nature 300, 713–718 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Capan, D. J., Chen, E. Y., Levinson, A. D., Seeburg, P. H. & Goeddel, D. V. Nature 302, 33–37 (1983).

    Article  ADS  Google Scholar 

  13. Wigler, M. et al. Cell 16, 777–785 (1980).

    Article  Google Scholar 

  14. Aiello, L., Guilfoyle, R., Huebner, K. & Weinman, R. Virology 94, 460–469 (1979).

    Article  CAS  PubMed  Google Scholar 

  15. Perricaudet, M., Akusjarvi, G., Virtanen, A. & Petterson, U. Nature 281, 694–696 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Langbeheim, H., Shih, T. Y. & Scolnick, E. M. Virology 106, 292–300 (1980).

    Article  CAS  PubMed  Google Scholar 

  17. Furth, M. E., Davis, L. J., Fleurdelys, B. & Scolnick, E. M. J. Virol. 43, 294–304 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Tabin, C. J. et al. Nature 300, 143–149 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  19. Taparowsky, E. et al. Nature 300, 762–765 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Reddy, E. P., Reynolds, R. K., Santos, E. & Barbacid, M. Nature 300, 149–152 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Shih, T. Y. et al. J. Virol. 42, 253–261 (1982).

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Wigler, M. et al. Proc. natn. Acad. Sci. U.S.A. 76, 1373–1376 (1979).

    Article  ADS  CAS  Google Scholar 

  23. Soeda, E., Arrand, J. R., Smolar, N., Walsh, J. & Griffin, B. E. Nature 283, 445–453 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Gingeras, T. et al. J. biol. Chem. 257, 13475–13491 (1982).

    CAS  PubMed  Google Scholar 

  25. Southern, E. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  26. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  PubMed  Google Scholar 

  27. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  28. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  29. Scolnick, E. M. & Parks, W. P. J. Virol. 13, 1211–1219 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Feramisco, J. R., Smart, J. E., Burridge, K. & Thomas, G. P. J. biol. Chem. 257, 11024–11031 (1982).

    CAS  PubMed  Google Scholar 

  31. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  32. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  33. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    Article  CAS  PubMed  Google Scholar 

  34. Land, H., Parada, L. F. & Weinberg, R. A. Nature 304, 596–602 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ruley, H. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature 304, 602–606 (1983). https://doi.org/10.1038/304602a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/304602a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing