Abstract
Short interspersed repetitive DNA sequences (SINEs) are the major component of dispersed repetitive DNA in all mammalian genomes (see refs 1–6 for reviews). Most SINEs contain an intragenic RNA polymerase III promoter that initiates transcription at the 5′ end of the repeated DNA sequence7–9 and which has been proposed to facilitate the transposition and amplification of these sequences by an RNA-intermediate mechanism10,11. We have discovered several SINE families in the prosimian Galago crassicaudatus which have promoter regions similar to transfer RNA genes12,13. To determine the relationship between Galago SINEs and mammalian tRNA genes, we have compared their sequences. Here, we demonstrate that the Galago monomer and type II SINE families12 are 68 and 62% homologous, respectively, with a human methionine tRNA gene14. We have extended our analysis to include the rat identifier15 and mouse B2 (ref. 16) families and show that their sequences are closely related to alanine and serine tRNA genes, respectively. Our observations suggest that many mammalian SINE families are amplified tRNA pseudogenes.
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Daniels, G., Deininger, P. Repeat sequence families derived from mammalian tRNA genes. Nature 317, 819–822 (1985). https://doi.org/10.1038/317819a0
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DOI: https://doi.org/10.1038/317819a0
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