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Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ

Abstract

Identification of the cellular proteins whose expression is regulated during the cell cycle in normal cells is essential for understanding the mechanisms involved in the control of cell proliferation. A nuclear protein called cyclin of relative molecular mass 36,000 (Mr 36K), whose synthesis correlates with the proliferative state of the cell, has been identified in several cell types of human, mouse, hamster and avian origin1–5. The rate of cyclin synthesis is very low in quiescent cells and increases several fold after serum stimulation shortly before DNA synthesis6,7. Immunofluorescence and autoradiography studies have shown that the nuclear staining patterns of cyclin during S phase have a sequential order of appearance and a clear correlation can be found between DNA synthesis and cyclin positive nuclei7–10. The proliferating cell nuclear antigen (PCNA)8,11–13 and cyclin have many common properties and it has been shown that these two are identical12,14. Recently a protein which is required by DNA polymerase-δ for its catalytic activity with templates having low primer/template ratios has been isolated from calf thymus15. We report here that cyclin and the auxiliary protein of DNA polymerase-δ are identical.

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References

  1. Bravo, R. & Graf, T. Expl Cell Res. 156, 450–454 (1985).

    Article  CAS  Google Scholar 

  2. Bravo, R., Celis, A., Mosses, D. & Celis, J. E. Cell Biol. int. Rep. 5, 479–489 (1981).

    Article  CAS  Google Scholar 

  3. Bravo, R. et al. Expl Cell Res. 136, 311–319 (1981).

    Article  CAS  Google Scholar 

  4. Bravo, R., Fey, S. J. & Celis, J. E. Carcinogenesis 2, 769–782 (1981).

    Article  CAS  Google Scholar 

  5. Bravo, R. Proc. natn. Acad. Sci. U.S.A. 81, 4848–4851 (1984).

    Article  ADS  CAS  Google Scholar 

  6. Bravo, R. & Macdonald-Bravo, H. EMBO J. 3, 3177–3181 (1984).

    Article  CAS  Google Scholar 

  7. Bravo, R. & Macdonald-Bravo, H. EMBO J. 4, 655–661 (1985).

    Article  CAS  Google Scholar 

  8. Takasaki, J., Deng, J. S. & Tan, E. M. J. exp. Med. 154, 1899–1909 (1981).

    Article  CAS  Google Scholar 

  9. Celis, A. & Celis, J. E. Proc. natn. Acad. Sci. U.S.A. 82, 3262–3266 (1985).

    Article  ADS  CAS  Google Scholar 

  10. Madsen, P. & Celis, J. E. FEBS Lett. 193, 5–11 (1985).

    Article  CAS  Google Scholar 

  11. Miyashi, K., Fritzler, M. J. & Tan, E. M. J. Immun. 121, 2228–2234 (1978).

    Google Scholar 

  12. Takasaki, J., Fischwild, D. & Tan, E. M. J. exp. Med. 159, 981–992 (1984).

    Article  CAS  Google Scholar 

  13. Tan, E. M. Adv. Immun. 33, 167–240 (1982).

    Article  CAS  Google Scholar 

  14. Malhews, M. B., Bernstein, R. M., Franza, R. R. & Garrels, J. I. Nature 309, 374–376 (1984).

    Article  ADS  Google Scholar 

  15. Tan, C. K., Castillo, C., So, A. G. & Downey, K. M. J. biol. Chem. 261, 12310–12316 (1986).

    Article  CAS  Google Scholar 

  16. Bravo, R. Expl Cell Res. 163, 287–293 (1986).

    Article  CAS  Google Scholar 

  17. Ogata, K., Ogata, Y., Nakamura, R. M. & Tan, E. M. J. Immun. 135, 2623–2677 (1985).

    CAS  PubMed  Google Scholar 

  18. Almendral, J., Hübsch, D., Blundell, P., Macdonald-Bravo, H. & Bravo, R. Proc. natn. Acad. Sci. U.S.A. (in the press).

  19. Bravo, R. & Celis, J. E. J. Cell Biol. 84, 795–802 (1980).

    Article  CAS  Google Scholar 

  20. Terasima, T. & Tolmach, L. J. Expl Cell Res. 30, 344–362 (1963).

    Article  CAS  Google Scholar 

  21. Macdonald-Bravo, H. & Bravo, R. Expl Cell Res. 156, 455–461 (1985).

    Article  CAS  Google Scholar 

  22. Kozu, T., Seno, T. & Yagura, T. Eur. J. Biochem. 157, 251–259 (1986).

    Article  CAS  Google Scholar 

  23. Thommes, R., Reiter, T. & Knippers, R. Biochemistry 25, 1308–1314 (1986).

    Article  CAS  Google Scholar 

  24. Fry, M. in Enzymes of Nucleic Acid Synthesis and Modification (ed. Jacob, S. T.) 39–92 (CRC, Boca Raton, 1983).

    Google Scholar 

  25. Chang, L. M. S. Science 191, 1183–1185 (1976).

    Article  ADS  CAS  Google Scholar 

  26. Bravo, R. in Two-dimensional Electrophoresis of Proteins (eds Celis, J. E. & Bravo, R.) 4–36 (Academic, New York, 1984).

    Google Scholar 

  27. Oppermann, H., Levinson, A. D., Varmus, H. E., Levintow, L. & Bishop, J. M. Proc. natn. Acad. Sci. U.S.A. 76, 1804–1808 (1979).

    Article  ADS  CAS  Google Scholar 

  28. Burnette, W. N. Analyt. Biochem. 112, 195–203 (1981).

    Article  CAS  Google Scholar 

  29. Gausepohl, H., Trosin, M. & Frank, R. in Advanced Methods in Protein Microsequence Analysis (eds Wittmann-Liebold, B. et al.) 149–160 (Springer, Heidelberg, 1986).

    Book  Google Scholar 

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Bravo, R., Frank, R., Blundell, P. et al. Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ. Nature 326, 515–517 (1987). https://doi.org/10.1038/326515a0

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