Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Rescue of bicoid mutant Drosophila embryos by Bicoid fusion proteins containing heterologous activating sequences

Abstract

The maternal gene bicoid (bed) determines pattern in the anterior half of the Drosophila embryo. It is reported here that the injection of bcd mutant embryos with messenger RNAs that encode proteins consisting of heterologous acidic transcriptional activating sequences fused to the DNA-binding portion of the bcd gene product, can completely restore the anterior pattern of the embryo.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Frohnhöfer, H. G. & Nüsslein-Volhard, C. Nature 324, 120–125 (1986).

    Article  ADS  Google Scholar 

  2. Driever, W. & Nüsslein-Volhard, C. Cell 54, 83–93 (1988).

    Article  CAS  Google Scholar 

  3. Frigerio, G., Burri, M., Bopp, D., Baumgartner, S. & Noll, M. Cell 47, 735–746 (1986).

    Article  CAS  Google Scholar 

  4. Berleth, T. et al. EMBO J. 7, 1749–1756 (1988).

    Article  CAS  Google Scholar 

  5. Driever, W. & Nusslein-Volhard, C. Cell 54, 95–104 (1988).

    Article  CAS  Google Scholar 

  6. Tautz, D. Nature 332, 281–284 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Schröder, C. et al. EMBO J. 7, 2881–2888 (1988).

    Article  Google Scholar 

  8. Lehmann, R. & Nüsslein-Volhard, C. Devl Biol. 119, 402–417 (1987).

    Article  CAS  Google Scholar 

  9. Tautz, D. et al. Nature 327, 383–389 (1987).

    Article  ADS  CAS  Google Scholar 

  10. Driever, W. & Nusslein-Volhard, C. Nature 337, 138–143 (1989).

    Article  ADS  CAS  Google Scholar 

  11. Driever, W., Thoma, G. & Nüsslein-Volhard, C. Nature 340, 363–367 (1989).

    Article  ADS  CAS  Google Scholar 

  12. McGinnis, W. et al. Nature 308, 428–433 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Laughon, A. & Scott, M. P. Nature 310, 25–31 (1984).

    Article  ADS  CAS  Google Scholar 

  14. Gehring, W. J. Science 236, 1245–1252 (1987).

    Article  ADS  CAS  Google Scholar 

  15. Desplan, C., Theis, J. & O'Farrell, P. Nature 318, 630–635 (1985).

    Article  ADS  CAS  Google Scholar 

  16. Hoey, T. & Levine, M. Nature 332, 858–861 (1988).

    Article  ADS  CAS  Google Scholar 

  17. Desplan, C., Theis, J. & O'Farrell, P. Cell 54, 1081–1090 (1988).

    Article  CAS  Google Scholar 

  18. Hanes, S. & Brent, R. Cell 57, 1275–1283 (1989).

    Article  CAS  Google Scholar 

  19. Jaynes, J. & O'Farrell, P. Nature 336, 744–749 (1988).

    Article  ADS  CAS  Google Scholar 

  20. Fitzpatrick, D. & Ingles, J. Nature 337, 666–668 (1989).

    Article  ADS  CAS  Google Scholar 

  21. Han, K., Levine, M. & Manley, J. Cell 56, 573–583 (1989).

    Article  CAS  Google Scholar 

  22. Struhl, G., Struhl, K. & Macdonald, P. Cell 57, 1259–1273 (1989).

    Article  CAS  Google Scholar 

  23. Brent, R. & Ptashne, M. Cell 43, 729–736 (1985).

    Article  CAS  Google Scholar 

  24. Keegan, L., Gill, G. & Ptashne, M. Science 231, 699–704 (1986).

    Article  ADS  CAS  Google Scholar 

  25. Ptashne, M. Nature 322, 697–701 (1986).

    Article  ADS  CAS  Google Scholar 

  26. Struhl, K. Cell 49, 295–297 (1987).

    Article  CAS  Google Scholar 

  27. Hope, I. & Struhl, K. Cell 46, 885–894 (1986).

    Article  CAS  Google Scholar 

  28. Ma, J. & Ptashne, M. Cell 48, 847–853 (1987).

    Article  CAS  Google Scholar 

  29. Ma, J. & Ptashne, M. Cell 51, 113–119 (1987).

    Article  CAS  Google Scholar 

  30. Giniger, E. & Ptashne, M. Nature 330, 670–672 (1987).

    Article  ADS  CAS  Google Scholar 

  31. Kakidani, H. & Ptashne, M. Cell 52, 161–167 (1988).

    Article  CAS  Google Scholar 

  32. Webster, N., Jin, J. R., Green, S., Hollis, M. & Chambon, P. Cell 52, 169–178 (1988).

    Article  CAS  Google Scholar 

  33. Fisher, J., Giniger, E., Ptashne, M. & Maniatis, T. Nature 332, 853–856 (1988).

    Article  ADS  Google Scholar 

  34. Ma, J., Przibilla, E., Hu, J., Bogorad, L. & Ptashne, M. Nature 334, 631–633 (1988).

    Article  ADS  CAS  Google Scholar 

  35. Courey, A. & Tjian, R. Cell 55, 887–898 (1988).

    Article  CAS  Google Scholar 

  36. Tora, L. et al. Cell (in the press).

  37. West, R., Yocum, R. & Ptashne, M. Molec. cell. Biol. 4, 2467–2478 (1984).

    Article  CAS  Google Scholar 

  38. Ammerer, G. Meth. Enzymol. 101, 192–201 (1983).

    Article  CAS  Google Scholar 

  39. Driever, W., Siegel, V. & Nüsslein-Volhard, C. Science (in the press).

  40. Triezenberg, S., Kingsbury, R. & McKnight, S. Genes Dev. 2, 718–729 (1988).

    Article  CAS  Google Scholar 

  41. Sadowski, I., Ma, J., Triezenberg, S. & Ptashne, M. Nature 335, 563–564 (1988).

    Article  ADS  CAS  Google Scholar 

  42. Gill, G. & Ptashne, M. Nature 334, 721–724 (1988).

    Article  ADS  CAS  Google Scholar 

  43. Ptashne, M. Nature 335, 683–689 (1988).

    Article  ADS  CAS  Google Scholar 

  44. Mlodzik, M. & Gehring, W. Development 101, 421–435 (1987).

    CAS  Google Scholar 

  45. Gaul, U. & Jäckle, H. Cell 51, 549–555 (1987).

    Article  CAS  Google Scholar 

  46. Gill, G. & Ptashne, M. Cell 51, 121–126 (1987).

    Article  CAS  Google Scholar 

  47. Yocum, R., West, R. & Ptashne, M. Molec. cell. Biol. 4, 1985–1998 (1984).

    Article  CAS  Google Scholar 

  48. Bunch, T. A., Grinblat, Y. & Goldstein, L. S. B. Nucleic Acids Res. 16, 1043–1061 (1988).

    Article  CAS  Google Scholar 

  49. Rio, D. C. & Rubin, G. M. Molec. cell. Biol. 5, 1833–1838 (1985).

    Article  CAS  Google Scholar 

  50. Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).

    Article  CAS  Google Scholar 

  51. Silver, P., Chiang, A. & Sadler, J. Genes Dev. 2, 707–717 (1988).

    Article  CAS  Google Scholar 

  52. Jürgens, G. et al. Wilhelm Roux's Arc. dev. Biol. 196, 141–157 (1986).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Driever, W., Ma, J., Nüsslein-Volhard, C. et al. Rescue of bicoid mutant Drosophila embryos by Bicoid fusion proteins containing heterologous activating sequences. Nature 342, 149–154 (1989). https://doi.org/10.1038/342149a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/342149a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing