Abstract
THE first instar larva of Drosophila consists of a chain of segments or parasegments1 in which the morphological pattern characteristic of each metaniere is determined by the homoeotic genes2–4, which are active in overlapping domains and are known to interact among themselves5–9. The interactions occur at the level of transcription and allow some homoeotic genes to control the patterns and levels of expression of others. The best known among them are the down-regulation of Antennapedia (Antp) by Ultrabithorax (Ubx) and that of Ubx by abdominal-A (abd-A) and Abdominal-B (Abd-B). It has been proposed7,10,11 that these cross-regulatory interactions play a part in specifying cell pattern, and hence the identity of each metaniere. Here we assess the functional significance of some of these interactions by expressing the Antp, Ubx or both homoeotic genes under the control of the heat-shock promoter12–14. Predictably, we find that homoeotic gene products evade normal regulatory controls and can be maximally expressed in regions where they are normally down-regulated, but, surprisingly, we find that interruption of the normal down-regulation of Antp and Ubx has no phenotypic consequences in the epidermis, where homoeotic phenotypes are normally manifest. Hence our results challenge the view that these, and possibly other cross-regulatory interactions have a role in determining segmentai identity.
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References
1. Martinez–Arias, A. & Lawrence, P. A. Nature 313, 639–642 (1985). 2. Lewis, E. B. Nature 276, 565–570 (1978). 3. SÃ nchez–Herrero, E., Vernos, l., Marco, R. & Morata, G. Nature 313, 108–113 (1985). 4. Wakimoto, B. & Kaufman, T. C. Devi Biol. 81, 51–64 (1981). 5. Struhl, G. Proc. nain. Acad. Sci. U.S.A. 79, 7380–7384 (1982). 6. Hafen, E., Levine, M. & Gehring, W. J. Nature 307, 287–289 (1984). 7. Struhl, G. & White, R. A. H. Cell 43, 507–519 (1985). 8. Bienz, M. & Tremml. G. Nature 333, 576–578 (1988). 9. Carroll, S. B., Laymon, R. A., McCutcheon, M. A., Riley, P. D. & Scott, M. P. Cell 47,113–122 (1986). 10. Beachy, P. A., Krasnow, M. A., Gavis, E. R. & Hogness, D. S. Celi 55, 1069–1081 (1988). 11. Peifer, M., Karch, F., Bender, W. Genes Dev. l, 891–898 (1987). 12. Schneuwly, S., Klemenz, R. & Gehring, W. J. Nature 325, 816–828 (1987). 13. Gibson, G. & Gehring, W. J. Development 102, 657–675 (1988). 14. Kuziora, M. A. & McGinnis, W. Cell 55, 477–485 (1988). 15. Struhl, G. Nature 308, 36–41 (1981). 16. Struhl, G. J. Embryo!, exp. Morph. 76, 297–331 (1983). 17. Mlodzik, M, Fjose, A. & Gehring, W. J. EMBO J. 2569–2578 (1988). 18. Mahaffey, J. W., Deiderich, R. J. & Kaufman, T. C. Development 105, 167–174 (1989). 19. Chadwick, R. & McGinnis, W. EMBO J. 6, 779–789 (1987). 20. Regulsky, M., McGinnis, N., Chadwick, R. & McGinnis, W. EMBO J. 6, 767–777 (1987). 21. LeMotte, P., Kuriowa, A., Fessier, L. I. & Gehring, W. EMBO J. 8, 219–227 (1989). 22. Krasnow, M. A., Saffman, E. E., Kornfeld, K. & Hogness, D. S. Cell 57, 1031–1043 (1989). 23. Wirz, J., Fessier, L. I. & Gehring, W. J. EMBO J. 5, 3327–3334 (1986). 24. Beachy, P. A., Helfand, S. L. & Hogness, D. S. Nature 313, 545–551 (1985). 25. Gehring, W. J. & Hiromi, Y. A. Rev. Genet. 20, 147–173 (1986). 26. Akam, M. Development 101, 1–22 (1987). 27. Muller, M. et al. EMBO J. 7, 4299–4304 (1988). 28. Struhl, G. Nature 338, 741–744 (1989).
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Gonzalez-Reyes, A., Urquia, N., Gehring, W. et al. Are cross-regulatory interactions between homoeotic genes functionally significant?. Nature 344, 78–80 (1990). https://doi.org/10.1038/344078a0
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DOI: https://doi.org/10.1038/344078a0
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