Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

BAX-dependent transport of cytochrome c reconstituted in pure liposomes

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: BAX-concentration-dependence of carboxyfluorescein dequenching and pore sizing by dextran inhibition.
Figure 2: BAX-dependent dequenching from FITC–dextran-containing liposomes.
Figure 3: Characterization of BAX-mediated release of cytochrome c.

References

  1. Gross, A., McDonnell, J. M. & Korsmeyer, S. J. Genes Dev. 13, 1899– 1911 (1999).

    Article  CAS  Google Scholar 

  2. Wolter, K. G. et al. J. Cell Biol. 139, 1281– 1292 (1997).

    Article  CAS  Google Scholar 

  3. Gross, A., Jockel, J., Wei, M. C. & Korsmeyer, S. J. EMBO J. 17, 3878–3885 (1998).

    Article  CAS  Google Scholar 

  4. Zou, H., Li, Y., Liu, X. & Wang, X. J. Biol. Chem. 274, 11549–15556 (1999).

    Article  CAS  Google Scholar 

  5. Finucane, D. M., Bossy-Wetzel, E., Waterhouse, N. J., Cotter, T. G. & Green, D. R. J. Biol. Chem. 274, 2225–2233 (1999).

    Article  CAS  Google Scholar 

  6. Pastorino, J. G., Chen, S. T., Tafani, M., Snyder, J. W. & Farber, J. L. J. Biol. Chem. 273, 7770– 7775 (1998).

    Article  CAS  Google Scholar 

  7. Xiang, J., Chao, D. T. & Korsmeyer, S. J. Proc. Natl Acad. Sci. USA 93, 14559–14563 (1996).

    Article  CAS  Google Scholar 

  8. Schlesinger, P. H. et al. Proc. Natl Acad. Sci. USA 94, 11357 –11362 (1997).

    Article  CAS  Google Scholar 

  9. Antonsson, B. et al. Science 277, 370– 372 (1997).

    Article  CAS  Google Scholar 

  10. Basanez, G. et al. Proc. Natl Acad. Sci. USA 96, 5492 –5497 (1999).

    Article  CAS  Google Scholar 

  11. Shimizu, S., Narita, M. & Tsujimoto, Y. Nature 399, 483– 487 (1999).

    Article  CAS  Google Scholar 

  12. Miller, C. Annu. Rev. Physiol. 46, 549–558 (1984).

    Article  CAS  Google Scholar 

  13. Chen, R. F. & Knutson, J. R. Anal. Biochem. 172 , 61–77 (1988).

    Article  CAS  Google Scholar 

  14. Priault, M., Chaudhuri, B., Clow, A., Camougrand, N. & Manon, S. Eur. J. Biochem. 260, 684– 691 (1999).

    Article  CAS  Google Scholar 

  15. Antonsson, B., Montessuit, S., Lauper, S., Eskes, R. & Martinou, J. C. Biochem. J. 345, 271–278 (2000).

    Article  CAS  Google Scholar 

  16. Schwarz, G., Zong, R. T. & Popescu, T. Biochim. Biophys. Acta. 1110, 97–104 (1992).

    Article  CAS  Google Scholar 

  17. Rex, S. Biophys. Chem. 58, 75–85 ( 1996).

    Article  CAS  Google Scholar 

  18. Schwarz, G. & Robert, C. H. Biophys. Chem. 42, 291–296 (1992).

    Article  CAS  Google Scholar 

  19. Schwarz, G. & Robert, C. H. Biophys. J. 58, 577 (1990).

    Article  CAS  Google Scholar 

  20. Dufourcq, J. et al. Biochim. Biophys. Acta. 859, 33– 48 (1986).

    Article  CAS  Google Scholar 

  21. Segel, I. H. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems 371–385 (John Wiley & Sons, New York, 1975).

  22. Renkin, E. M. J. Gen. Physiol. 38, 225 (1954 ).

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Levitt, D. G. Curr. Top. Membr. Transp. 21, 181 ( 1984).

    Article  CAS  Google Scholar 

  24. Hille, B. J. Gen. Physiol. 51, 199–219 (1968).

    Article  CAS  Google Scholar 

  25. Badley, R. A. in Modern Fluorescence Spectroscopy 112–119 (Plenum, New York, 1976).

  26. Eftink, M. R. & Ghiron, C. A. Anal. Biochem. 114 , 199–227 (1981).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported in part by the Spencer T. and Ann W. Olin Foundation.

Correspondence and requests for materials should be addressed to P.H.S. Supplementary Information is available on Nature Cell Biology’s World-Wide Web site (http://www.nature.com/ncb) or as paper copy from the London editorial office of Nature Cell Biology.

Author information

Authors and Affiliations

Authors

Supplementary information

Supplementary Figure 1

Diffusion constants determined by dynamic light scattering. (PDF 47 kb)

Supplementary Table 1 

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saito, M., Korsmeyer, S. & Schlesinger, P. BAX-dependent transport of cytochrome c reconstituted in pure liposomes. Nat Cell Biol 2, 553–555 (2000). https://doi.org/10.1038/35019596

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/35019596

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing