Abstract
Sulphate-reducing microbes affect the modern sulphur cycle, and may be quite ancient1,2, though when they evolved is uncertain. These organisms produce sulphide while oxidizing organic matter or hydrogen with sulphate3. At sulphate concentrations greater than 1 mM, the sulphides are isotopically fractionated (depleted in 34S) by 10–40‰ compared to the sulphate, with fractionations decreasing to near 0‰ at lower concentrations2,4,5,6. The isotope record of sedimentary sulphides shows large fractionations relative to seawater sulphate by 2.7 Gyr ago, indicating microbial sulphate reduction7. In older rocks, however, much smaller fractionations are of equivocal origin, possibly biogenic but also possibly volcanogenic2,8,9,10. Here we report microscopic sulphides in ∼3.47-Gyr-old barites from North Pole, Australia, with maximum fractionations of 21.1‰, about a mean of 11.6‰, clearly indicating microbial sulphate reduction. Our results extend the geological record of microbial sulphate reduction back more than 750 million years, and represent direct evidence of an early specific metabolic pathway—allowing time calibration of a deep node on the tree of life.
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References
Wagner, M., Roger, A. J., Flax, J. L., Brusseau, G. A. & Stahl, D. A. Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. J. Bacteriol. 180, 2975–2982 (1998).
Canfield, D. E. & Raiswell, R. The evolution of the sulfur cycle. Am. J. Sci. 299, 697–723 (1999).
Postgate, J. R. The Sulphate-Reducing Bacteria 2nd edn (Cambridge Univ. Press, Cambridge, 1984).
Harrison, A. G. & Thode, H. G. Mechanisms of the bacterial reduction of sulphate from isotope fractionation studies. Trans. Faraday Soc. 53, 84–92 (1958).
Kaplan, I. R. & Rittenberg, S. C. Microbiological fractionation of sulphur isotopes. J. Gen. Microbiol. 34, 195–212 (1964).
Knoll, A. H. & Canfield, D. E. Isotopic inferences on early ecosystems. Paleontol. Soc. Pap. 4, 212–243 (1998).
Goodwin, A. M., Monster, J. & Thode, H. G. Carbon and sulfur isotope abundances in Archean iron-formations and early Precambrian life. Econ. Geol. 71, 870–891 (1976).
Cameron, E. M. Sulphate and sulphate reduction in early Precambrian. Nature 296, 145–148 (1982).
Hayes, J. M., Lambert, I. B. & Strauss, H. in The Proterozoic Biosphere: A Multidisciplinary Study (eds Schopf, J. W. & Klein, C.) 129–134 (Cambridge Univ. Press, Cambridge, 1992).
Ohmoto, H., Kakegawa, T. & Lowe, D. R. 3.4-billion-year-old biogenic pyrites from Barberton, South Africa: sulfur isotope evidence. Science 262, 555–557 (1993).
Buick, R. & Dunlop, J. S. R. Evaporitic sediments of early Archaean age from the Warrawoona Group, North Pole, Western Australia. Sedimentology 37, 247–277 (1990).
Buick, R. et al. Record of emergent continental crust ∼3.5 billion years ago in the Pilbara Craton of Australia. Nature 375, 574–577 (1995).
Buick, R. & Barnes, K. R. Cherts in the Warrawoona Group: early Archaean silicified sediments deposited in shallow water environments. Univ. West. Aust. Geol. Dept Univ. Extension Spec. Publ. 9, 37–53 (1984).
Lambert, I. B., Donnelly, T. H., Dunlop, J. S. R. & Groves, D. I. Stable isotope compositions of early Archaean sulphate deposits of probable evaporite and volcanogenic origins. Nature 276, 808–810 (1978).
Groves, D. I., Dunlop, J. S. R. & Buick, R. An early habitat of life. Sci. Am. 245, 64–73 (1981).
Nijman, W., de Bruijne, K. C. H. & Valkering, M. E. Growth fault control of Early Archaean cherts, barite mounds and chert-barite veins, North Pole Dome, Eastern Pilbara, Western Australia. Precambr. Res. 88, 25–52 (1999).
Rankin, A. H. & Shepherd, T. J. H2S-bearing fluid inclusions in baryte from the North Pole deposit, Western Australia. Mineral. Mag. 42, 408–410 (1978).
Ohmoto, H. & Goldhaber, M. B. in Geochemistry of Hydrothermal Ore Deposits (ed. Barnes, H. L.) 517–611 (Wiley, New York, 1997).
Cameron, E. M. & Hattori, K. Archean gold mineralization and oxidized hydrothermal fluids. Econ. Geol. 82, 1177–1191 (1987).
Hardie, L. A. The gypsum-anhydrite equilibrium at one atmosphere pressure. Am. Mineral. 52, 171–200 (1967).
Canfield, D. E., Habicht, K. S. & Thamdrup, B. The Archean sulfur cycle and the early history of atmospheric oxygen. Science 288, 658–661 (2000).
Schidlowski, M. A 3800-million-year isotopic record of life from carbon in sedimentary rocks. Nature 333, 313–318 (1988).
Rosing, M. T. 13C-depleted carbon microparticles in >3700-Ma sea-floor sedimentary rocks from west Greenland. Science 283, 674–676 (1999).
Schopf, J. W. & Packer, B. M. Early Archean (3.3-billion to 3.5-billion-year-old) microfossils from the Warrawoona Group, Australia. Science 237, 70–73 (1987).
Widdel, F. in Biology of Anaerobic Microorganisms (ed. Zehnder, A. J. B.) 496–585 (Wiley, New York, 1988).
Stetter, K. O. in Evolution of Hydrothermal Ecosystems on Earth (and Mars?) (eds Bock, G. R. & Goode, J. A.) 1–10 (Wiley, New York, 1996).
Pace, N. R. A molecular view of microbial diversity and the biosphere. Science 276, 734–740 (1997).
Canfield, D. E., Raiswell, R., Westrich, J. T., Reaves, C. M. & Berner, R. A. The use of chromium reduction in the analysis of reduced inorganic sulfur in sediments and shales. Chem. Geol. 54, 149–155 (1986).
Canfield, D. E. A new model for Proterozoic ocean chemistry. Nature 396, 450–453 (1998).
Knoll, A. H. A new molecular window on early life. Science 285, 1025–1026 (1999).
Acknowledgements
We thank J. S. R. Dunlop for suggesting that we should examine the isotopic systematics of microscopic sulphur species in the North Pole barite; K.-U. Hinrichs, K. Londry, R. Summons, B. Thamdrup and K. Habicht for discussions; I. O'Brien, O. Thomas and L. Salling for technical assistance; and D. Des Marais for comments and suggestions. This work was supported by the Danish Grundforkningsfond (Basic Research Foundation) and by the Australian Research Council (R.B.).
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Shen, Y., Buick, R. & Canfield, D. Isotopic evidence for microbial sulphate reduction in the early Archaean era. Nature 410, 77–81 (2001). https://doi.org/10.1038/35065071
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DOI: https://doi.org/10.1038/35065071
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