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  • Review Article
  • Published:

Transmembrane crosstalk between the extracellular matrix and the cytoskeleton

Nature Reviews Molecular Cell Biology volume 2, pages 793–805 (2001)Cite this article

Key Points

  • Cell adhesions represent the interaction interfaces between cells and the extracellular matrix. Their study provides exciting insights into the interplay between physical forces and molecular signalling in cell regulation.

  • Cells use transmembrane actin–integrin adhesion complexes as mechanosensors to probe the rigidity of the extracellular environment, mediate adhesion, trigger signalling, and remodel the extracellular matrix (ECM).

  • Local physical forces induce transitions in the types and functions of these cell–matrix adhesions: Focal complexes transform into focal adhesions, which serve as the source of fibrillar adhesions.

  • Integrin translocation appears to stretch fibronectin molecules, exposing cryptic sites that mediate matrix assembly into extracellular fibrils.

  • A key mechanism in these transitions appears to be conformational changes induced by force or a local reorganization of scaffold or signalling molecules to promote multimolecular assembly.

  • Both intracellular molecular-complex formation at adhesion sites and ECM assembly are regulated by Rho-family GTPases.

  • The main challenges for the future include:

     The identification of the full repertoire of adhesion-associated molecules.

     Comparison of the various 'focal complex'-like structures.

     Characterization of the molecular and cellular nature of the mechanosensors involved in cell–matrix adhesion.

     Characterization of the regulation and functional integration of the various forms of adhesions, which change depending on the state of differentiation, tissue location, and application of local forces.

     Exploring the structure and function of cell–matrix adhesions in three-dimensional microenvironments in vivo and explaining the roles of complex carbohydrates in cell–matrix interactions.

Abstract

Integrin-mediated cell adhesions provide dynamic, bidirectional links between the extracellular matrix and the cytoskeleton. Besides having central roles in cell migration and morphogenesis, focal adhesions and related structures convey information across the cell membrane, to regulate extracellular-matrix assembly, cell proliferation, differentiation, and death. This review describes integrin functions, mechanosensors, molecular switches and signal-transduction pathways activated and integrated by adhesion, with a unifying theme being the importance of local physical forces.

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Figure 1: Immunofluorescence microscopic localization of the main forms of integrin-mediated matrix adhesions.
Figure 2: Schematic depicting the complexity of the main molecular domains of cell–matrix adhesions.
Figure 3: Cells probe, respond to, and remodel the extracelluar matrix (ECM) using integrin–actin cytoskeleton adhesion complexes.
Figure 4: Modulation of focal-adhesion assembly by application of external force (a–d) or relaxation of actomyosin contractility (e–g).
Figure 5: Cells reorganize the underlying substrate differentially depending on its composition.
Figure 6: Ligand-binding regions and interaction sites of fibronectin.
Figure 7: Schematic representation of the main steps in fibronectin fibrillogenesis.

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Acknowledgements

Figure 1a and b were kindly provided by E. Zamir, figure 1c by I. Grosheva and Figure 1d by J. Blair. B.G. is the incumbent of the E. Neter Chair in Cell and Tumor Biology, A.B. holds the J. Moss Chair of Biomedical Research.

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Authors and Affiliations

  1. Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Benjamin Geiger & Alexander Bershadsky

  2. Craniofacial Developmental Biology and Regeneration Branch, National Institute of Craniofacial and Dental Research, National Institutes of Health, Bethesda, 20892, MD, USA

    Roumen Pankov & Kenneth M. Yamada

Authors
  1. Benjamin Geiger
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  2. Alexander Bershadsky
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  3. Roumen Pankov
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  4. Kenneth M. Yamada
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Correspondence to Benjamin Geiger.

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DATABASES

Interpro:

SH2 domain

 Locuslink:

14-3-3-β

α-actinin

ASAP1

calpain II

caveolin

dynamin

fibronectin

filamin

ILK

α4 integrin

α9 integrin

LAR

mDia1

PAK

PI3K

ponsin

ROCK

tensin

uPAR

VASP/Ena

 Swiss-Prot:

caldesmon

DOCK180

DRAL

dystroglycan

gelsolin

GRAF

α3 integrin

α5 integrin

α6 integrin

αv integrin

β1 integrin

β3 integrin

layilin

paxillin

SHIP-2

SHPS-1

SHP-2

syndecan-4

talin

vinculin

vinexin

vitronectin

Glossary

INTEGRINS

A group of heterodimeric transmembrane adhesion receptors for extracellular-matrix proteins such as fibronectin and vitronectin.

BASEMENT MEMBRANE

A dense, sheet-like, laminated extracellular matrix that separates epithelia, muscle, or other tissues from connective tissue.

LAMELLIPODIUM

A thin, flat extension at the cell periphery, which is filled with a branching meshwork of actin filaments.

RHO-FAMILY GTPASES

A family of monomeric G proteins — comprising Rho, Rac and Cdc42 — that are homologous to Ras. These are important molecular switches, which control cytoskeletal assembly and contraction.

MACROPHAGE

A white blood cell that is specialized for phagocytosis.

OSTEOCLAST

A specialized cell that is involved in active bone resorption.

FIBRONECTIN MODULES

Subunits of fibronectin are comprised of repeating structural modules of three types (I, II, III). Each module is encoded by one or two exons with introns that precisely separate repeats. There are 12 type I modules, each around 45 amino-acids long and clustered into three groups; two type 2 modules, each 60 amino acids-long; and 15–17 type III repeats, each about 90 amino-acids long (see Fig. 6).

BDM

(2,3-Butanedione monoxime) An inhibitor of myosin ATPase.

ML-7

(1-(5-iodonaphthalene-1-sulphonyl)-1-H-hexahydro-1, 4-diazepine) A kinase inhibitor thought to be relatively specific for myosin light-chain kinase.

H-7

(1-(5-isoquinolinylsulphonyl)-2-methylpiperazine) A broad-spectrum serine–threonine kinase inhibitor that blocks myosin light-chain kinase, Rho kinase and certain other kinases.

LASER TWEEZERS

Microscope-based device that traps micron-sized particles in a focused laser beam. Can be used to move or to stop such particles.

LEADING EDGE

The leading region of the advancing lamellipodium in a motile cell.

CELL-INDUCED SUBSTRATE WRINKLING

An approach for visualizing cellular contractility that is based on wrinkling of a thin and flexible silicone rubber film on which the cell is cultured.

MICRO-CANTILEVER TILTING DEVICE

A microscopic device in which cells are attached to a surface that consists of arrays of cantilevers. Local forces that are applied to this surface induce tilting of these cantilevers, which can be measured.

DEFORMATION OF ELASTIC GELS

Polymeric elastic gels that either contain impregnated beads or are surface micro-patterned are used as substrates for cultured cells. Local forces that are applied to these substrates can be measured, based on the distortion of these patterns.

CONNECTIVE TISSUES

Tissues that form the architectural framework of the vertebrate body. In these tissues, the extracellular matrix is plentiful and cells are sparsely distributed within it.

GRANULATION TISSUE

A contractile, myofibroblast-containing tissue formed in wounds.

ISOMETRIC TENSION

A condition in which contraction of muscle, non-muscle cells or the actomyosin network is opposed by an equal load that prevents net shortening, even though tension increases.

LATRUNCULIN-A

A macrolide that is derived from the Red Sea sponge Latrunculia magnifica, which binds and sequesters actin molecules, and thereby prevents the assembly of actin filaments.

TREADMILLING

A special state in polymer dynamics, when monomer addition at one end occurs at the same rate as monomer dissociation at the other end, which keeps the polymer length unchanged.

BARBED END

The fast-polymerizing end of actin filaments (defined by the arrowhead-shaped decoration of actin filaments with myosin fragments).

STEERED MOLECULAR DYNAMICS SIMULATION

A computer simulation method for studying force-induced reactions in biopolymers.

RGD ADHESION SEQUENCE

The primary adhesive motif in many extracellular matrix molecules, which contains the amino-acid triplet, Arg–Gly–Asp.

TRANSGLUTAMINASE

An enzyme (such as factor XIIIa) that helps to crosslink fibronectin and other molecules through isopeptide linkages.

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Geiger, B., Bershadsky, A., Pankov, R. et al. Transmembrane crosstalk between the extracellular matrix and the cytoskeleton. Nat Rev Mol Cell Biol 2, 793–805 (2001). https://doi.org/10.1038/35099066

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