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ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor

Nature volume 367, pages 525–532 (1994)Cite this article

Abstract

Genetic control elements are usually situated in local regions of chromatin that are hypersensitive to structural probes such as DNase I. We have reconstructed the chromatin structure of the hsp70 promoter using an in vitro nucleosome assembly system. Binding of the GAGA transcription factor on existing nucleosomes leads to nucleosome disruption, DNase I hypersensitivity at the TATA box and heat-shock elements, and rearrangement of adjacent nucleosomes. ATP hydrolysis facilitates this process, suggesting that an energy-dependent pathway is involved in chromatin remodelling.

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References

  1. Felsenfeld, G. Nature 335, 219–224 (1992).

    Article  ADS  Google Scholar 

  2. Grunstein, M. Rev. Cell. Biol. 6, 643–678 (1990).

    Article  CAS  Google Scholar 

  3. Kornberg, R. D. & Lorch, Y. A. Rev. Cell. Biol. 8, 563–587 (1992).

    Article  CAS  Google Scholar 

  4. Wu, C. Nature 286, 854–860 (1980).

    Article  ADS  CAS  Google Scholar 

  5. Gross, D. S. & Garrard, W. T. A. Rev. Biochem. 57, 159–197 (1988).

    Article  CAS  Google Scholar 

  6. Workman, J. L. & Buchman, A. R. Trends biochem. Sci. 18, 90–95 (1993).

    Article  CAS  Google Scholar 

  7. Hayes, J. J. & Wolffe, A. P. Bioessays 14, 597–603 (1992).

    Article  CAS  Google Scholar 

  8. Becker, P. B. & Wu, C. Molec. cell. Biol. 12, 2241–2249 (1992).

    Article  ADS  CAS  Google Scholar 

  9. Lis, J. T. & Wu, C. Cell 74, 1–4 (1993).

    Article  CAS  Google Scholar 

  10. Soeller, W. & Kornberg, T. Molec. cell. Biol. 13, 7961–7970 (1993).

    Article  CAS  Google Scholar 

  11. Biggin, M. & Tjian, R. Cell 53, 699–711 (1988).

    Article  CAS  Google Scholar 

  12. Dynlacht, B. D., Hoey, T. & Tjian, R. T. Cell 66, 563–576 (1991).

    Article  CAS  Google Scholar 

  13. Rougvie, A. E. & Lis, J. T. Cell 54, 795–804 (1988).

    Article  CAS  Google Scholar 

  14. Rasmussen, E. & Lis, J. T. Proc. natn. Acad. Sci. U.S.A. 90, 7923–7927 (1993).

    Article  ADS  CAS  Google Scholar 

  15. Gilmour, D. S., Thomas, G. H. & Elgin, S. C. R. Science 245, 1487–1490 (1989).

    Article  ADS  CAS  Google Scholar 

  16. Wu, C. Nature 317, 84–87 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Purnell, B. A. & Gilmour, D. S. Molec. cell. Biol. 13, 2593–2603 (1993).

    Article  CAS  Google Scholar 

  18. Parker, C. S. & Topol, J. Cell 36, 357–369 (1984).

    Article  CAS  Google Scholar 

  19. van Holde, K. E. Chromatin 16–30 (Springer, New York, 1989).

    Google Scholar 

  20. Wu, C. Nature 309, 229–234 (1984).

    Article  ADS  CAS  Google Scholar 

  21. Elgin, S. C. R. & Hood, L. E. J. biol. Chem. 12, 4984–4991 (1973).

    CAS  Google Scholar 

  22. Blackman, R. K. & Meselson, M. J. molec. Biol. 188, 499–515 (1986).

    Article  CAS  Google Scholar 

  23. Ashburner, M. in Developmental Studies on Giant Chromosomes (Springer, Berlin, 1972).

    Google Scholar 

  24. Burtis, K. C. et al. Cell 61, 85–89 (1990).

    Article  CAS  Google Scholar 

  25. Lue, Q., Wallrath, L. L., Granok, H. & Eigin, S. C. R. Molec. cell. Biol. 13, 2802–2814 (1993).

    Article  Google Scholar 

  26. Kerrigan, L. A., Croston, G. E., Lira, L. & Kadonaga, J. T. J. biol. Chem. 266, 574–582 (1991).

    CAS  PubMed  Google Scholar 

  27. Harrison, S. D. & Travers, A. A. EMBO J. 9, 207–216 (1990).

    Article  CAS  Google Scholar 

  28. Read, D. & Manley, J. L. EMBO J. 11, 1035–1044 (1992).

    Article  CAS  Google Scholar 

  29. Brown, J. L. & Wu, C. Development 117, 45–58 (1993).

    CAS  PubMed  Google Scholar 

  30. DiBello, P. R. et al. Genetics 129, 385–397 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  31. Xue, F. & Cooley, L. Cell 72, 681–693 (1993).

    Article  CAS  Google Scholar 

  32. Peterson, C. L. & Herskowitz I. Cell 68, 573–583 (1992).

    Article  CAS  Google Scholar 

  33. Winston, F. & Carlson, M. Trends Genet. 8, 387–391 (1992).

    Article  CAS  Google Scholar 

  34. Henikoff, S. Trends biochem. Sci 18, 291–292 (1993).

    Article  CAS  Google Scholar 

  35. Tamkun, J. W. et al. Cell 68, 561–572 (1992).

    Article  CAS  Google Scholar 

  36. Almouzni G. & Wolffe, A. Expl Cell Res. 205, 1–15 (1993).

    Article  CAS  Google Scholar 

  37. Schmid, A., Fascher, K. D. & Horz W. Cell 71, 1–20 (1992).

    Article  Google Scholar 

  38. Chasman, D. I. et al. Genes Dev. 4, 503–514 (1990).

    Article  CAS  Google Scholar 

  39. Morrow, B. E., Ju, Q. & Warner, J. R. Molec. cell. Biol. 13, 1173–1182 (1993).

    Article  CAS  Google Scholar 

  40. Devlin, C., Tice-Baldwin, K., Shore, D. & Arndt K. T. Molec. cell. Biol. 11, 3642–3651 (1991).

    Article  CAS  Google Scholar 

  41. Axelrod, J. D., Reagan, M. S. & Majors, J. Genes Dev. 7, 857–869 (1993).

    Article  CAS  Google Scholar 

  42. Workman, J. L. & Kingston, R. E. Science 258, 1780–1784 (1992).

    Article  ADS  CAS  Google Scholar 

  43. Workman, J. L., Taylor, I. A. C. & Kingston R. E. Cell 64, 533–544 (1991).

    Article  CAS  Google Scholar 

  44. Kamakaka, R. T., Bulger, M. & Kadonaga, J. T. Genes Dev. 7, 1779–1795 (1993).

    Article  CAS  Google Scholar 

  45. Lorch, Y., LaPointe, J. W. & Kornberg, R. D. Genes Dev. 6, 2282–2287 (1992).

    Article  CAS  Google Scholar 

  46. Perlman, T. & Wragne, O. EMBO J. 7, 3073–3083 (1988).

    Article  Google Scholar 

  47. Pina, B., Bruggemeier, u. & Beato, M. Cell 60, 719–731 (1990).

    Article  CAS  Google Scholar 

  48. Archer, T. K. et al. Molec. cell. Biol. 11, 688–698 (1991).

    Article  CAS  Google Scholar 

  49. Mason, P. J. et al. J. molec. Biol. 156, 21–35 (1982).

    Article  CAS  Google Scholar 

  50. Croston, G. E., Lira, L. M. & Kadonaga, J. T. Prot. Expr. Purific 2, 162–169 (1991).

    Article  CAS  Google Scholar 

  51. Westwood, J. T., Clos, J., & Wu, C. Nature 353, 822–827 (1991).

    Article  ADS  CAS  Google Scholar 

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Author notes

  1. Peter B. Becker

    Present address: Gene Expression Programme, EMBL, Meyerhofstrasse 1, D-69117, Heidel-berg, Germany

Authors and Affiliations

  1. Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Building 37, Room 4C-09, Bethesda, Maryland, 20892, USA

    Toshio Tsukiyama, Peter B. Becker & Carl Wu

Authors
  1. Toshio Tsukiyama
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  2. Peter B. Becker
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  3. Carl Wu
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Tsukiyama, T., Becker, P. & Wu, C. ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor. Nature 367, 525–532 (1994). https://doi.org/10.1038/367525a0

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