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The crystal structure of the bacterial chaperonln GroEL at 2.8 Å

Nature volume 371, pages 578–586 (1994)Cite this article

Abstract

The crystal structure of Escherichia coli GroEL shows a porous cylinder of 14 subunits made of two nearly 7-fold rotationally symmetrical rings stacked back-to-back with dyad symmetry. The subunits consist of three domains: a large equatorial domain that forms the foundation of the assembly at its waist and holds the rings together; a large loosely structured apical domain that forms the ends of the cylinder; and a small slender intermediate domain that connects the two, creating side windows. The three-dimensional structure places most of the mutation-ally defined functional sites on the channel walls and its outward imaginations, and at the ends of the cylinder.

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Author information

Author notes

  1. Zbyszek Otwinowski

    Present address: Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Mines Blvd, Dallas, Texas, 75235-9038, USA

  2. Rashmi Hegde

    Present address: Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, New York, 10016, USA

  3. Andrzej Joachimiak

    Present address: Argonne National Laboratory, 9700 Cass Avenue, Argonne, Illinois, 60439-4833, USA

Authors and Affiliations

  1. Department of Genetics, Yale University School of Medicine, Boyer Center, 295 Congress Avenue, New Haven, Connecticut, 06510, USA

    Kerstin Braig, David C. Boisvert & Arthur L. Horwich

  2. Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, Boyer Center, 295 Congress Avenue, New Haven, Connecticut, 06510, USA

    Zbyszek Otwinowski, Rashmi Hegde, Andrzej Joachimiak & Paul B. Sigler

  3. Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center, 295 Congress Avenue, New Haven, Connecticut, 06510, USA

    Kerstin Braig, Zbyszek Otwinowski, Rashmi Hegde, Arthur L. Horwich & Paul B. Sigler

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Braig, K., Otwinowski, Z., Hegde, R. et al. The crystal structure of the bacterial chaperonln GroEL at 2.8 Å. Nature 371, 578–586 (1994). https://doi.org/10.1038/371578a0

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