Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A protein related to extracellular matrix proteins deleted in the mouse mutant reeler

Abstract

THE autosomal recessive mouse mutation reeler1–3 leads to impaired motor coordination, tremors and ataxia4. Neurons in affected mice fail to reach their correct locations in the developing brain, disrupting the organization of the cerebellar and cerebral cortices and other laminated regions5–8. Here we use a previously characterized reeler allele (rltg)9 to clone a gene, reelin, deleted in two reeler alleles. Normal but not mutant mice express reelin in embryonic and postnatal neurons during periods of neuronal migration. The encoded protein resembles extracellular matrix proteins involved in cell adhesion. The reeler phenotype thus seems to reflect a failure of early events associated with brain lamination which are normally controlled by reelin.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Dernoncourt, C., Ruelle, D. & Goffinet, A. M. Genomics 11, 1167–1169 (1991).

    Article  CAS  PubMed  Google Scholar 

  2. Bar, I. et al. Genomics (in the press).

  3. Montgomery, J. C., Guamieri, M. H., Tartaglia, K. E. & Flaherty, L. A. Mamm. Genome (in the press).

  4. Falconer, D. S. J. Genet. 50, 192–201 (1951).

    Article  CAS  PubMed  Google Scholar 

  5. Hamburgh, M. Devl Biol. 8, 165–185 (1963).

    Article  CAS  Google Scholar 

  6. Mariani, J., Crepel, F., Mikoshiba, K., Changeux, J. P. & Sotelo, C. Phil. Trans. R. Soc. B281, 1–28 (1977).

    Article  CAS  Google Scholar 

  7. Caviness, V. S. J. & Rakic, P. A. Rev. Neurosci. 1, 297–326 (1978).

    Article  Google Scholar 

  8. Goffinet, A. M. Int. J. devl Biol. 36, 101–107 (1992).

    CAS  Google Scholar 

  9. Miao, G. G. et al. Proc. natn. Acad. Sci. U.S.A. 91, 11050–11054 (1994).

    Article  ADS  CAS  Google Scholar 

  10. Buckler, A. J. et al. Proc. natn. Acad. Sci. U.S.A. 88, 4005–4009 (1991).

    Article  ADS  CAS  Google Scholar 

  11. Kozak, M. J.Cell Biol. 115, 887–903 (1991).

    Article  CAS  PubMed  Google Scholar 

  12. Heijne, V. J. Molec. Biol. 173, 243–251 (1984).

    Article  Google Scholar 

  13. Bourdon, M. A., Krusius, T., Campbell, S., Schwartz, N. B. & Ruoslahti, E. Proc. natn. Acad. Sci. U.SA. 84, 3194–3198 (1987).

    Article  ADS  CAS  Google Scholar 

  14. Klar, A., Baldassare, M. & Jessel, T. M. Cell 69, 95–110 (1992).

    Article  CAS  PubMed  Google Scholar 

  15. Engel, J. FEBS Lett. 251, 1–7 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Goffinet, A. M. Brain Res. 319, 261–296 (1984).

    Article  CAS  PubMed  Google Scholar 

  17. Derer, P. & Nakanishi, S. J. Hirnforsch. 24, 209–224 (1983).

    CAS  PubMed  Google Scholar 

  18. Godfraind, C., Schachner, M. & Goffinet, A. M. Brain Res. 470, 99–111 (1988).

    Article  CAS  PubMed  Google Scholar 

  19. Stanfield, B. B. & Cowan, W. M. J. comp. Neurol. 185, 461–484 (1979).

    Article  CAS  PubMed  Google Scholar 

  20. Terashima, T., Inoue, K., lnoue, Y., Yokoyama, M. & Mikoshiba, K. J. comp. Neurol. 252, 264–278 (1986).

    Article  CAS  PubMed  Google Scholar 

  21. Guenet, J.-L. Mouse News Lett. 41, (1981).

  22. Flaherty, L., Messer, A., Russell, L. B. & Rinchik, E. M. Proc. natn. Acad. Sci. U.S.A. 89, 2859–2863 (1992).

    Article  ADS  CAS  Google Scholar 

  23. Franco, B. et al. Nature 353, 529–536 (1991).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Legouis, R. et al. Cell 67, 423–435 (1991).

    Article  CAS  PubMed  Google Scholar 

  25. Mischel, P. S., Nguyen, L. P. & Vinters, H. V. J. Neuropath. exp. Neurol. 54, 137–153 (1995).

    Article  CAS  PubMed  Google Scholar 

  26. Xanthoudakis, S., Miao, G., Wang, F., Pan, Y.-C. E. & Curran, T. EMBO J. 11, 3323–3335 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Cathala, G. et al. DNA 2, 329–335 (1983).

    Article  CAS  PubMed  Google Scholar 

  28. Lugo, D. I., Roberts, J. L. & Pintar, J. E. Molec. Endocrin. 3, 1313–1324 (1989).

    Article  CAS  Google Scholar 

  29. Simmons, D. M., Arriza, J. L. & Swanson, L. W. J. Histotechn. 12, 169–181 (1989).

    Article  CAS  Google Scholar 

  30. Ogawa, et al. Neurons (in the press).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

D'Arcangelo, G., G. Miao, G., Chen, SC. et al. A protein related to extracellular matrix proteins deleted in the mouse mutant reeler. Nature 374, 719–723 (1995). https://doi.org/10.1038/374719a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/374719a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing