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Isolation of a hyperthermophilic archaeum predicted by in situ RNA analysis

Abstract

A variety of hyperthermophilic bacteria and archaea1 have been isolated from high-temperature environments by plating and serial dilutions2. However, these techniques allow only the small percentage of organisms able to form colonies, or those that are predominant within environmental samples, to be obtained in pure culture. Recently, in situ 16S ribosomal RNA analyses of samples from the Obsidian hot pool at Yellowstone National Park, Wyoming, revealed a variety of archaeal sequences, which were all different from those of previously isolated species3,4. This suggests substantial diversity of archaea with so far unknown morphological, physiological and biochemical features, which may play an important part within high-temperature ecosystems. Here we describe a procedure to obtain pure cultures of unknown organisms harbouring specific 16S rRNA sequences identified previously within the environment. It combines visual recognition of single cells by phylogenetic staining5–9 and cloning by 'optical tweezers'10,11. Our result validates polymerase chain reaction data on the existence of large archaeal communities.

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References

  1. Woese, C. R. Kandler, O. & Wheelis, M. L. Proc. natn. Acad. Sci. U.S.A. 87, 4576–4579 (1990).

    Article  ADS  CAS  Google Scholar 

  2. Blöchl, E. et al. Wld J. Microbiol. Biotechnol. 11, 9–16 (1995).

    Article  Google Scholar 

  3. Barns, S. M., Fundyga, R. E., Jeffries, M. W. & Pace, N. R. Proc. natn. Acad. Sci. U.S.A. 91, 1609–1613 (1994).

    Article  ADS  CAS  Google Scholar 

  4. Barns, S. M., Fundyga, R. E., Jeffries, M. W. & Pace, N. R. Abstr. 94th Gen. Meet. Am. Soc. Microbiol. 1994, 253 (1994).

  5. DeLong, E. F., Wickham, G. S. & Pace, N. R. Science 243, 1360–1363 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Amann, R. I., Krumholz, L. & Stahl, D. A. J. Bact. 172, 762–770 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Stahl, D. A. & Amann, R. I. in Sequencing and Hybridization Techniques in Bacterial Systematics (eds Stackebrandt, E. & Goodfellow, M.) 205–248 (Wiley, Chichester, 1991).

    Google Scholar 

  8. Kane, M. D., Poulsen, L. K. & Stahl, D. A. Appl. envir. Microbiol. 59, 682–686 (1993).

    CAS  Google Scholar 

  9. Burggraf, S. et al. Appl. envir. Microbiol. 60, 3112–3119 (1994).

    CAS  Google Scholar 

  10. Ashkin, A., Dziedzic, J. M. & Yamane, T. Nature 330, 769–771 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Ashkin, A. & Dziedzic, J. M. Science 235, 1517–1520 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Brosius, J., Palmer, J. L., Kennedy, J. P. & Noller, H. F. Proc. natn. Acad. Sci. U.S.A. 75, 4801–4805 (1978).

    Article  ADS  CAS  Google Scholar 

  13. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R. & Wolfe, R. S. Microbiol. Rev. 43, 260–296 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Stetter, K. O. et al. Zentbl. Bakt. Hyg., I. Abt. Orig. C2, 166–178 (1981).

    CAS  Google Scholar 

  15. Stetter, K. O. Nature 300, 258–260 (1982).

    Article  ADS  Google Scholar 

  16. Stetter, K. O., Lauerer, G., Thomm, M. & Neuner, A. Science 236, 822–824 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Huber, R., Kurr, M., Jannasch, H. W. & Stetter, K. O. Nature 342, 833–834 (1989).

    Article  ADS  Google Scholar 

  18. Huber, R. & Stetter, K. O. in The Procaryotes (eds Balows, A., Trüper, H. G. Dworkin, M., Harder, W. & Schleifer, K.-H.) 677–683 (Springer, New York, 1992).

    Google Scholar 

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Huber, R., Burggraf, S., Mayer, T. et al. Isolation of a hyperthermophilic archaeum predicted by in situ RNA analysis. Nature 376, 57–58 (1995). https://doi.org/10.1038/376057a0

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