Abstract
THE Rev protein of human immunodeficiency virus type 1 (HIV-1) facilitates the nuclear export of unspliced and partly spliced viral RNAs. Rev contains an RNA binding domain, required for interaction with HIV-1 RNA, and an effector domain, required for RNA-bound Rev to function. The Rev effector domain is believed to interact with a cellular cofactor required for the Rev response and thus HIV-1 replication1,2. Here we report the use of a yeast two-hybrid screen to clone human Rev interacting protein (hRIP), which specifically interacts with the Rev effector domain. This hRIP protein has homology with nucleoporins, a class of proteins that mediate nucleocytoplasmic transport3,4. These and other properties of hRIP are those expected of a Rev cellular cofactor.
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Fritz, C., Zapp, M. & Green, M. A human nucleoporin-like protein that specifically interacts with HIV Rev. Nature 376, 530–533 (1995). https://doi.org/10.1038/376530a0
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DOI: https://doi.org/10.1038/376530a0
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