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Positional cloning of the mouse retrovirus restriction gene Fvl

Abstract

VERTEBRATE evolution has taken place against a background of constant retrovirus infection, and much of the mammalian genome consists of endogenous retrovirus-like elements1. Several host genes have evolved to control retrovirus replication2, including Friend-virus-susceptibility-1, Fv1, on mouse chromosome 4 (refs 3, 4). The Fv1 gene acts on murine leukaemia virus at a stage after entry into the target cell but before integration and formation of the provirus5. Although restriction is not absolute, Fv1 prevents or delays spontaneous or experimentally induced viral tumours2. In vitro, Fv1 restriction leads to an apparent 50–1,000 fold reduction in viral titre6. Genetic evidence implicates a direct interaction between the Fv1 gene product and a component of the viral preintegration complex, the capsid protein CA (refs 7–9). We have now cloned Fv1: the gene appears to be derived from the gag region of an endogenous retrovirus unrelated to murine leukaemia virus, implying that the Fv1 protein and its target may share functional similarities despite the absence of nucleotide-sequence homology.

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References

  1. Baltimore, D. Cell 40, 481–482 (1985).

    Article  CAS  Google Scholar 

  2. Steeves, R. & Lilly, F. Annu. Rev. Genet. 11, 277–296 (1977).

    Article  CAS  Google Scholar 

  3. Lilly, F. J. Natl Cancer Inst. 45, 163–169 (1970).

    CAS  PubMed  Google Scholar 

  4. Rowe, W. P., Humphrey, J. B. & Lilly, F. J. Exp. Med. 137, 850–853 (1973).

    Article  CAS  Google Scholar 

  5. Pryciak, P. M. & Varmus, H. E. J. Virol. 66, 5959–5966 (1992).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Hartley, J. W., Rowe, W. P. & Huebner, R. J. J. Virol. 5, 221–225 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Boone, L. R., Innes, C. L., Glover, P. L. & Linney, E. J. Virol. 63, 2592–2957 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Bowerman, B., Brown, P. O., Bishop, J. M. & Varmus, H. E. Genes Dev. 3, 469–478 (1989).

    Article  CAS  Google Scholar 

  9. Rommelaere, J., Donis-Keller, H. & Hopkins, N. Cell 16, 43–50 (1979).

    Article  CAS  Google Scholar 

  10. Frankel, W. N., Stoye, J. P., Taylor, B. A. & Coffin, J. M. J. Virol. 63, 1763–1774 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Stoye, J. P., Kaushik, N. Jeremiah, S. & Best, S. Mammalian Genome 6, 31–36 (1995).

    Article  CAS  Google Scholar 

  12. Jolicoeur, P. Curr. Top. Microbiol. Immunol. 86, 67–122 (1979).

    Article  CAS  Google Scholar 

  13. Lander, M. R. & Chattopadhyay, S. K. J. Virol. 52, 695–698 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Pincus, T., Hartley, J. W. & Rowe, W. P. Virology 65, 333–342 (1975).

    Article  CAS  Google Scholar 

  15. Guigo, R., Knudsen, S., Drake, N. & Smith, T. J. Mol. Biol. 226, 141–157 (1992).

    Article  CAS  Google Scholar 

  16. Cordonnier, A., Casella, J.-F. & Heidmann, T. J. Virol. 69, 5890–5897 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Kozak, C. A. & O'Neill, R. R. J. Virol. 61, 3082–3088 (1987).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Kozak, C. A. J. Virol. 55, 281–285 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Gardner, M. B., Kozak, C. A. & O'Brien, S. J. Trends Genet. 7, 22–27 (1991).

    Article  CAS  Google Scholar 

  20. Burke, D. T., Rossi, J. M., Koos, D. S. & Tilghman, S. M. Mammalian Genome 1, 65 (1991).

    Article  CAS  Google Scholar 

  21. Chartier, F. L. et al. Nature Genet. 1, 132–136 (1992).

    Article  CAS  Google Scholar 

  22. Srivastava, A. K. & Schlessinger, D. Gene 103, 53–59 (1991).

    Article  CAS  Google Scholar 

  23. Pachnis, V., Pevny, L., Rothstein, R. & Constantini, F. Proc. Natl Acad. Sci. USA 87, 5109–5113 (1990).

    Article  ADS  CAS  Google Scholar 

  24. Rein, A. Virology 120, 251–257 (1982).

    Article  CAS  Google Scholar 

  25. Duran-Troise, G., Bassin, R. H., Wallace, B. F. & Rein, A. Virology 112, 795–799 (1981).

    Article  CAS  Google Scholar 

  26. Holland, C. A., Wozney, J., Chatis, P. A., Hopkins, N. & Hartley, J. W. J. Virol. 53, 152–157 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Boone, L. R. et al. J. Virol. 48, 110–119 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Morgenstern, J. P. & Land, H. Nucleic Acids Res. 18, 3587–3596 (1990).

    Article  CAS  Google Scholar 

  29. Goff, S., Traktman, P. & Baltimore, D. J. Virol. 38, 239–248 (1981).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Morgan, B. A. et al. Proc. Natl Acad. Sci. USA 93, 2801–2806 (1996).

    Article  ADS  CAS  Google Scholar 

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Best, S., Tissier, P., Towers, G. et al. Positional cloning of the mouse retrovirus restriction gene Fvl. Nature 382, 826–829 (1996). https://doi.org/10.1038/382826a0

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