Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • In the Laboratory
  • Published:

An economic method for the fluorescent labeling of PCR fragments

Abstract

A poor man's approach to genotyping for research and high-throughput diagnostics.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Amplification scheme for the one-tube, single-reaction nested PCR method.
Figure 2: (A) The informative pedigree of a family in which the MaoA locus on the X chromosome was haplotyped.

References

  1. Litt, M. & Luty, J.A. Am. J. Hum. Genet. 44 , 397–401 (1989).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Smith, L.M. et al. Nature 321, 674–679 ( 1986).

    Article  CAS  Google Scholar 

  3. Black, G.C.M., Chen, Z.Y., Craig, I.W. & Powell, J.F. Nucleic Acids. Res. 19, 689 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Ju, J., Glazer, A.N. & Mathies, R.A. Nat. Med. 2, 246– 249 (1996).

    Article  CAS  Google Scholar 

  5. Hung, S.C., Mathies, R.A. & Glazer, A.N. Anal. Biochem. 252, 78– 88 (1997).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schuelke, M. An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18, 233–234 (2000). https://doi.org/10.1038/72708

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/72708

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing