Abstract
We describe a protein with the hallmarks of a chemokine, designated CXCL16, that is made by dendritic cells (DCs) in lymphoid organ T cell zones and by cells in the splenic red pulp. CXCL16 contains a transmembrane domain and both membrane-bound and soluble forms are produced. Naïve CD8 T cells, natural killer T cells and a subset of memory CD4 T cells bind CXCL16, and activated T cells migrated chemotactically to the soluble chemokine. By expression cloning, Bonzo (also known as STRL33 and TYMSTR) was identified as a CXCL16 receptor. CXCL16 may function in promoting interactions between DCs and CD8 T cells and in guiding T cell movements in the splenic red pulp. CXCL16 was also found in the thymic medulla and in some nonlymphoid tissues, indicating roles in thymocyte development and effector T cell trafficking.
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Acknowledgements
We thank P. Hyman for technical help, P. Lane for the Fc expression plasmid, C. Turck for protein sequencing, A. Weiss for helpful input and L. Mintz for comments on the manuscript. Supported by NIH grant AI45073 and Packard Foundation (to J. G. C.) and UCSF Molecular Medicine Training Program and NIH Academic Rheumatology and Clinical Immunology training grant AR07304 (to M. M.).
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Matloubian, M., David, A., Engel, S. et al. A transmembrane CXC chemokine is a ligand for HIV-coreceptor Bonzo. Nat Immunol 1, 298–304 (2000). https://doi.org/10.1038/79738
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DOI: https://doi.org/10.1038/79738
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