Abstract
In the course of our screening program for regulators of a molecular chaperone GRP78 expression, we isolated a novel inhibitor of GRP78 expression, designated as prunustatin A, from Streptomyces violaceoniger 4521-SVS3. The structure of prunustatin A was determined by a series of NMR analyses to be an oxidized type of the neoantimycin family. Prunustatin A inhibited the expression of GRP78 induced by 2-deoxyglucose in human fibrosarcoma HT1080 cells accompanied by global cell death without showing cytotoxicity under normal nutrient condition.
Similar content being viewed by others
Article PDF
References
Katschinski DM, Jacobson EL, Wiedemann GJ, Robins HI . Modulation of VP-16 cytotoxicity by carboplation and 41.8°C hyperthermia. Cancer Res Clin Oncol 127: 425–435 ( 2001)
Imaizumi K, Miyoshi K, Katayama T, Yoneda T, Taniguchi M, Kubo T, Tohyama M . The unfolded protein response and Alzheimer's disease. Biochim Biophys Acta 1536: 85–96 ( 2001)
Kaufman RJ . Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev 13: 1211–1233 ( 1999)
Park HR, Furihata K, Hayakawa Y, Shin-ya K . Versipelostatin, a novel GRP78/Bip molecular chaperone down-regulator of microbial origin. Tetrahedron Lett 43: 6941–6945 ( 2002)
Caglioti L, Misiti D, Mondelli R, Selva A, Arcamone F, Cassinelli G . The structure of neoantimycin. Tetrahedron 25: 2193–2221 ( 1969)
Watanabe K, Tanaka T, Fukuhara K, Miyairi N, Yonehara H, Umezawa H . Blastmycin: a new antibiotic from Streptomyces sp. J Antibiot 10A: 39–45 ( 1957)
Kinoshita M, Wada M, Umezawa S . The total syntheses of a diastereomeric mixture of antimycin A3 (blastmycin). J Antibiot 22: 580–582 ( 1969)
Takahashi K, Tsuda E, Kurosawa K . SW-163A and B, novel immunosuppressants produced by Streptomyces sp. J Antibiot 54: 867–873 ( 2001)
Park HR, Tomida A, Sato S, Tsukumo Y, Yun J, Yamori T, Hayakawa Y, Tsuruo T, Shin-ya K . Blockade of survival response to glucose deprivation for selective killing of tumor cells. J Natl Can Inst 96: 1300–1330 ( 2004)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Umeda, Y., Chijiwa, S., Furihata, K. et al. Prunustatin A, a Novel GRP78 Molecular Chaperone Down-regulator Isolated from Streptomyces violaceoniger. J Antibiot 58, 206–209 (2005). https://doi.org/10.1038/ja.2005.25
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/ja.2005.25
Keywords
This article is cited by
-
Indolylkojyl methane analogue IKM5 potentially inhibits invasion of breast cancer cells via attenuation of GRP78
Breast Cancer Research and Treatment (2019)
-
JBIR-52, a new antimycin-like compound, from Streptomyces sp. ML55
The Journal of Antibiotics (2009)
-
A Novel Antimycin-like Compound, JBIR-06, from Streptomyces sp. ML55
The Journal of Antibiotics (2008)
-
Efrapeptin J, a New Down-regulator of the Molecular Chaperone GRP78 from a Marine Tolypocladium sp.
The Journal of Antibiotics (2008)
-
A Novel Versipelostatin Analogue, Versipelostatin F Isolated from Streptomyces versipellis 4083-SVS6
The Journal of Antibiotics (2008)