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Expression profiling of persistent polyclonal B-cell lymphocytosis suggests constitutive expression of the AP-1 transcription complex and downregulation of Fas-apoptotic and TGFβ signalling pathways

Leukemia volume 23, pages 581–583 (2009)Cite this article

Persistent polyclonal B-cell lymphocytosis (PPBL), first described in 1982, is a rare apparently benign lymphoproliferative disorder mainly occurring in middle-aged female smokers that is characterized by elevated serum polyclonal immunoglobulin M levels and persistent lymphocytosis of often binucleated B cells. PPBL is often associated with chromosomal abnormalities including +i3(q), premature chromosome condensation and BCL2/Ig gene rearrangements, and linked with HLA-DR7 expression.1 The molecular basis of this disorder however remains largely unexplored.

Therefore, we used whole genome microarray expression analysis to elucidate the transcriptome of 14 patients diagnosed with PPBL in comparison with 6 control samples from healthy donors (4 individuals and 2 pools (12 donors each)). PPBL patients (10 women, 4 men) had a median age of 43 (range 28–63). All were smokers with an average lymphocyte count of 5.34 × 107 per ml (range 3.7–9). Individual patient details are given in Supplementary Table S1. Total RNA (100 ng), purified from Trizol, was amplified and labelled using the one-cycle method and hybridized to GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA, USA) as recommended by the manufacturers.

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Acknowledgements

This work was funded by grants from the Leukaemia Research Fund (CHL, HC, JB, JSW), the Julian Starmer-Smith Memorial Trust (CHL) and Cancer Research UK (FP).

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Authors and Affiliations

  1. Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK

    C H Lawrie, R Shilling, H Cattan, F Pezzella, J Boultwood & J S Wainscoat

  2. Laboratoire d′Hématologie, CHU de Caen, Caen, France

    X Troussard

  3. Déparement de Genetique, Laboratoire Pasteur, Cerba, France

    H Mossafa

  4. Department of Haematology, John Radcliffe Hospital, Oxford, UK

    C S R Hatton

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  1. C H Lawrie
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  2. R Shilling
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Correspondence to C H Lawrie.

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Supplementary Information accompanies the paper on the Leukemia website (http://www.nature.com/leu)

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Lawrie, C., Shilling, R., Troussard, X. et al. Expression profiling of persistent polyclonal B-cell lymphocytosis suggests constitutive expression of the AP-1 transcription complex and downregulation of Fas-apoptotic and TGFβ signalling pathways. Leukemia 23, 581–583 (2009). https://doi.org/10.1038/leu.2008.223

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