Abstract
Eukaryotic cells depend on cytoskeletal polymers and molecular motors to establish their asymmetrical shapes, to transport intracellular constituents and to drive their motility. Cell biologists are using diverse experimental approaches to understand the molecular basis of cellular movements and to explain why defects in the component proteins cause disease. Much of the molecular machinery for motility evolved in early eukaryotes, so a limited set of general principles can explain the motility of most cells.
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References
Pollard, T. D. & Borisy, G. G. Cellular motility driven by assembly and disassembly of actin filaments. Cell 112, 453–465 (2003).
Mohler, P. J. et al. Ankyrin-B mutation causes type 4 long-QT cardiac arrhythmia and sudden cardiac death. Nature 421, 634–639 (2003).
Gerull, B. et al. Mutations of TTN, encoding the giant muscle filament titin, cause familial dilated cardiomyopathy. Nature Genet. 30, 201–204 (2002).
Hu, A., Wang, F. & Sellers, J. R. Mutations in human nonmuscle myosin IIA found in patients with May-Hegglin anomaly and Fechtner syndrome result in impaired enzymatic function. J. Biol. Chem. 277, 46512–46517 (2002).
van den Ent, F., Amos, L. A. & Lowe, J. Bacterial ancestry of actin tubulin. Curr. Opin. Microbiol. 634–638 (2001).
Møller-Jensen, J., Jensen, R. B., Löwe, J. & Gerdes, K. Prokaryotic DNA segregation by an actin-like filament. EMBO J. 21, 3119–3127 (2002).
Roberts, T. M. & Stewart, M. Acting like actin. The dynamics of the nematode major sperm protein (msp) cytoskeleton indicate a push-pull mechanism for amoeboid cell motility. J. Cell Biol. 149, 7–12 (2000).
Fuchs, E. & Cleveland, D. W. A structural scaffolding of intermediate filaments in health and disease. Science 279, 514–519 (1998).
Mocz, G. & Gibbons, I. R. Model for the motor component of dynein heavy chain based on homology to the AAA family of oligometric ATPases. Structure 9, 93–103 (2001).
Vale, R. D. & Milligan, R. A. The way things move: looking under the hood of molecular motors proteins. Science 288, 88–95 (2000).
Berg, J. S., Powell, B. C. & Cheney, R. E. A millennial myosin census. Mol. Biol. Cell 780–794 (2001).
Tong, A. H. et al. A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules. Science. 295, 321–324 (2002).
Tong, A. H. et al. Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294, 2364–2368 (2001).
Robinson, R. C. et al. Crystal structure of Arp2/3 complex. Science 294, 1660–1661 (2001).
Kim, A. S., Kakalis, L. T., Abdul-Manan, N., Liu, G. A. & Rosen, M. K. Autoinhibition and activation mechanisms of the Wiskott–Aldrich syndrome protein. Nature 404, 151–158 (2000).
Volkman, B. F., Prehoda, K. E., Scott, J. A., Peterson, F. C. & Lim, W. A. Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Syndrome. Cell 111, 565–576 (2002).
Li, H., DeRosier, D. J., Nicholson, W. V., Nogales, E. & Downing, K. H. Microtubule structure at 8 A resolution. Structure. 10, 1317–1328 (2002).
Burgess, S. A., Walker, M. L., Sakakibara, H., Knight, P. J. & Oiwa, K. Dynein structure and power stroke. Nature 421, 715–718 (2003).
Waterman-Storer, C. M., Desai, A., Bulinski, J. C. & Salmon, E. D. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr. Biol. 8, 1227–1230 (1998).
Watanabe, N. & Mitchison, T. J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295, 1083–1086 (2002).
Wang, L. & Brown, A. Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching. Mol. Biol. Cell 12, 3257–3267 (2001).
Gerbal, F., Chaikin, P., Rabin, Y. & Prost, J. An elastic analysis of Listeria monocytogenes propulsion. Biophys J. 79, 2259–2275 (2000).
Loisel, T. P., Boujemaa, R., Pantaloni, D. & Carlier, M. F. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins. Nature 401, 613–616 (1999).
Walker, R. A. et al. Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. J. Cell Biol. 107, 1437–1448 (1988).
Amann, K. J. & Pollard, T. D. Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection microscopy. Proc. Natl Acad. Sci. USA 98, 15009–15013 (2001).
Peterson, J. R. & Mitchison, T. J. Small molecules, big impact. A history of chemical inhibitors and the cytoskeleton. Chem. Biol. 9, 1275–1285 (2002).
Bray, D. Bacterial chemotaxis and the question of gain. Proc. Natl Acad. Sci. USA 99, 7–9 (2002).
Tyson, J. J., Chen, K. & Novak, B. Network dynamics and cell physiology. Nature Rev. Mol. Cell Biol. 2, 908–916 (2001).
Mogilner, A. & Edelstein-Keshet, L. Regulation of actin dynamics in rapidly moving cells: a quantitative analysis. Biophys. J. 83, 1237–1258 (2002).
Roy, P. et al. Local photorelease of caged thymosin β4 in locomoting keratocytes causes cell turning. J. Cell Biol. 153, 1035–1048 (2002).
Pollard, T. D. & Earnshaw, W. C. Cell Biology (W. B. Saunders, New York, 2002).
Pollard, T. D., Blanchoin, L. & Mullins, R. D. Biophysics of actin filament dynamics in nonmuscle cells. Annu. Rev. Biophys. Biomol. Struct. 29, 545–576 (2000).
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Pollard, T. The cytoskeleton, cellular motility and the reductionist agenda. Nature 422, 741–745 (2003). https://doi.org/10.1038/nature01598
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DOI: https://doi.org/10.1038/nature01598
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