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A curated compendium of phosphorylation motifs

Nature Biotechnology volume 25, pages 285–286 (2007)Cite this article

To the editor:

Protein phosphorylation is a covalent post-translational modification event that is essential for regulation and maintenance of most biological processes in eukaryotes1. Conventionally, phosphorylation sites have been discovered using biochemical experiments with the sequence contexts or 'motifs' generally defined by manually aligning the sequences of phosphorylated peptides. Today, high-throughput methods, such as tandem mass spectrometry, allow rapid and directed discovery of hundreds of phosphorylation sites in a single experiment, which necessitates analysis of the 'motifs' in the identified phosphopeptides. Until now, however, there has been no single resource that allows investigators to determine whether a protein/peptide of interest contains a motif that has been previously described in the literature. We describe below a publicly available catalog of phosphorylation motifs that we have created for the community (http://www.hprd.org/PhosphoMotif_finder).

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Acknowledgements

This work was supported by a contract N01-HV-28180 from the National Heart Lung and Blood Institute and grants U54RR020839 and TR01CA106424T from the National Institutes of Health. We thank Shashikanth for assistance with the curation.

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Authors and Affiliations

  1. McKusick-Nathans Institute of Genetic Medicine and the Departments of Biological Chemistry, Oncology and Pathology, Johns Hopkins University, Baltimore, 21205, Maryland, USA

    Ramars Amanchy, Balamurugan Periaswamy, Suresh Mathivanan, Raghunath Reddy & Akhilesh Pandey

  2. Institute of Bioinformatics, International Tech Park, Bangalore, 560 066, India

    Balamurugan Periaswamy, Suresh Mathivanan, Raghunath Reddy & Sudhir Gopal Tattikota

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  1. Ramars Amanchy
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  2. Balamurugan Periaswamy
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Supplementary information

Supplementary Table 1

A list of phosphotyrosine motifs. (PDF 68 kb)

Supplementary Table 2

A list of phosphoserine/threonine motifs. (PDF 72 kb)

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Amanchy, R., Periaswamy, B., Mathivanan, S. et al. A curated compendium of phosphorylation motifs. Nat Biotechnol 25, 285–286 (2007). https://doi.org/10.1038/nbt0307-285

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