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Expression of a Barley Ribosome-Inactivating Protein Leads to Increased Fungal Protection in Transgenic Tobacco Plants

Abstract

We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato wun1 gene. R0 transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phyto-pathogen Rhizoctonia solani. Three R0 plants, which grew well compared to controls, and which were shown to carry the transgene by Southern blotting, were selfed and the R1 progeny tested for protection against fungal inoculation. Southern and 2-D Western blotting showed that R1 plants exhibiting increased protection had inherited the transgene and expressed the barley RIP upon wounding.

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Logemann, J., Jach, G., Tommerup, H. et al. Expression of a Barley Ribosome-Inactivating Protein Leads to Increased Fungal Protection in Transgenic Tobacco Plants. Nat Biotechnol 10, 305–308 (1992). https://doi.org/10.1038/nbt0392-305

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