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Expression of Amplified Hepatitis B Virus Surface Antigen Genes in Chinese Hamster Ovary Cells

Bio/Technology volume 3, pages 561–566 (1985)Cite this article

Abstract

Mammalian cell lines which synthesize and secrete hepatitis B surface antigen (HBsAg) at levels of up to 15 μg/106 cells/day have been obtained by introducing hepatitis B virus eoding sequences into hamster cells together with dihydrofolate reductase cDNA and selecting methotrexate resistant clones. The immunogenic potential of the HBsAg particles tested in guinea pigs is identical to those from human serum. The particles carry, like Dane particles, a receptor for polymerized human serum albumin and may constitute a particularly efficient vaccine.

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Author notes

  1. Rolf E. Streeck: To whom correspondence should be addressed.

Authors and Affiliations

  1. Groupement de Génie Génétique, Institut Pasteur, 28 rue du Docteur Roux, 75724, Paris, Cedex 15, France

    Maire-Louise Michel, Eliane Sobczak, Yves Malpièce & Rolf E. Streeck

  2. Unité de Recombinaison et Expression Génétique (INSERM U.163, CNRS LA 271), Institut Pasteur, 28 rue du Docteur Roux, 75724, Paris, Cedex 15, France

    Maire-Louise Michel & Pierre Tiollais

Authors
  1. Maire-Louise Michel
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  2. Eliane Sobczak
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  3. Yves Malpièce
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  4. Pierre Tiollais
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  5. Rolf E. Streeck
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Michel, ML., Sobczak, E., Malpièce, Y. et al. Expression of Amplified Hepatitis B Virus Surface Antigen Genes in Chinese Hamster Ovary Cells. Nat Biotechnol 3, 561–566 (1985). https://doi.org/10.1038/nbt0685-561

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  • DOI: https://doi.org/10.1038/nbt0685-561

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