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Antibody-targeted Photolysis of Bacteria In Vivo

Bio/Technology volume 12, pages 703–706 (1994)Cite this article

Abstract

We have evaluated the efficacy of antibody-targeted photolysis to kill bacteria in vivo using specific antibacterial photosensitizer (PS) immunoconjugates. After infecting the dorsal skin in mice with Pseudomonas aeruginosa, both specific and nonspecific tin (IV) chlorin e6-monoclonal antibody conjugates were injected at the infection site. After a 15 min incubation period, the site was exposed to 630 nm light with a power density of 100 mW/cm2 for 1600 seconds. Irradiation resulted in a greater than 75% decrease in the number of viable bacteria at sites treated with a specific conjugate, whereas normal bacterial growth was observed in animals that were untreated or treated with a nonspecific conjugate. Antibody-targeted photolysis may be a selective and versatile tool for treating a variety of infections.

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Author notes

  1. Martin L. Yarmush: Corresponding author.

Authors and Affiliations

  1. Surgical Services, Massachusetts General Hospital, and the Shriners Burns Institute, Boston, MA, 02114

    Francois Berthiaume, Steven R. Reiken, Mehmet Toner, Ronald G. Tompkins & Martin L. Yarmush

  2. Department of Chemical and Biochemical Engineering, Center for Advanced Biotechnology and Medicine, Rutgers University, P.O. Box 909, Piscataway, NJ, 08854

    Martin L. Yarmush

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  1. Francois Berthiaume
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  4. Ronald G. Tompkins
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  5. Martin L. Yarmush
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Berthiaume, F., Reiken, S., Toner, M. et al. Antibody-targeted Photolysis of Bacteria In Vivo. Nat Biotechnol 12, 703–706 (1994). https://doi.org/10.1038/nbt0794-703

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