Abstract
The organization of secretory traffic remains unclear, mainly because of the complex structure and dynamics of the secretory pathway. We have thus studied a simplified system, a single synchronized traffic wave crossing an individual Golgi stack, using electron tomography. Endoplasmic-reticulum-to-Golgi carriers join the stack by fusing with cis cisternae and induce the formation of intercisternal tubules, through which they redistribute their contents throughout the stack. These tubules seem to be pervious to Golgi enzymes, whereas Golgi vesicles are depleted of both enzymes and cargo. Cargo then traverses the stack without leaving the cisternal lumen. When cargo exits the stack, intercisternal connections disappear. These findings provide a new view of secretory traffic that includes dynamic intercompartment continuities as key players.
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Acknowledgements
A. Mironov and A. Luini are principal investigators and have contributed equally to this project. We would like to thank all those who provided us with antibodies and cDNAs, P. Lupetti for assistance with fast-freezing experiments, C. P. Berrie for critical reading of the manuscript and E. Fontana for artwork preparation. We acknowledge financial support from the AIRC, Telethon Italy and a European Research Training Network (A.L. and K.N.J.B.). A.J.K. is supported by the Royal Netherlands Academy of Arts and Sciences (KNAW), and W.J.C.G. and K.N.J.B. by FEI.
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Trucco, A., Polishchuk, R., Martella, O. et al. Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments. Nat Cell Biol 6, 1071–1081 (2004). https://doi.org/10.1038/ncb1180
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DOI: https://doi.org/10.1038/ncb1180
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