The biology of RNA interference has greatly facilitated analysis of loss-of-function phenotypes, but correlating these phenotypes with small-molecule inhibition profiles is not always straightforward. We examine the rationale of comparing RNA interference to pharmacological intervention in chemical biology.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis
Nature Communications Open Access 25 June 2020
-
Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours
BMC Cancer Open Access 23 April 2020
-
Focal Adhesion Kinase Inhibition Contributes to Tumor Cell Survival and Motility in Neuroblastoma Patient-Derived Xenografts
Scientific Reports Open Access 13 September 2019
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Kim, D.H. & Rossi, J.J. Nat. Rev. Genet. 8, 173–184 (2007).
Walensky, L.D. et al. Science 305, 1466–1470 (2004).
Knight, Z.A. & Shokat, K.M. Chem. Biol. 12, 621–637 (2005).
Carmena, M. & Earnshaw, W.C. Nat. Rev. Mol. Cell Biol. 4, 842–854 (2003).
Andrews, P.D. Oncogene 24, 5005–5015 (2005).
Keen, N. & Taylor, S. Nat. Rev. Cancer 4, 927–936 (2004).
Matthews, N., Visintin, C., Hartzoulakis, B., Jarvis, A. & Selwood, D.L. Expert Rev. Anticancer Ther. 6, 109–120 (2006).
Ditchfield, C. et al. J. Cell Biol. 161, 267–280 (2003).
Hauf, S. et al. J. Cell Biol. 161, 281–294 (2003).
Biggins, S. & Murray, A.W. Genes Dev. 15, 3118–3129 (2001).
Ruchaud, S., Carmena, M. & Earnshaw, W.C. Nat. Rev. Mol. Cell Biol. 8, 798–812 (2007).
Girdler, F. et al. J. Cell Sci. 119, 3664–3675 (2006).
Barr, A.R. & Gergely, F. J. Cell Sci. 120, 2987–2996 (2007).
Manfredi, M.G. et al. Proc. Natl. Acad. Sci. USA 104, 4106–4111 (2007).
Marumoto, T. et al. J. Biol. Chem. 278, 51786–51795 (2003).
Engelman, J.A., Luo, J. & Cantley, L.C. Nat. Rev. Genet. 7, 606–619 (2006).
Fan, Q.W. et al. Cancer Cell 9, 341–349 (2006).
Knight, Z.A. et al. Cell 125, 733–747 (2006).
Pu, P., Kang, C., Zhang, Z., Liu, X. & Jiang, H. Technol. Cancer Res. Treat. 5, 271–280 (2006).
An, H.J. et al. J. Pathol. 212, 161–169 (2007).
Ueki, K. et al. Mol. Cell. Biol. 22, 965–977 (2002).
Ueki, K. et al. J. Biol. Chem. 278, 48453–48466 (2003).
Luo, J. & Cantley, L.C. Cell Cycle 4, 1309–1312 (2005).
Seidler, J., McGovern, S.L., Doman, T.N. & Shoichet, B.K. J. Med. Chem. 46, 4477–4486 (2003).
Jackson, A.L. et al. Nat. Biotechnol. 21, 635–637 (2003).
Meraldi, P., Honda, R. & Nigg, E.A. EMBO J. 21, 483–492 (2002).
Acknowledgements
S.S.T. is a Cancer Research UK Senior Fellow. The authors acknowledge support from the Howard Hughes Medical Institute (K.M.S.), the US National Institutes of Health (K.M.S. and W.A.W.), the Wallace H. Coulter, Pediatric Brain Tumor and Samuel G. Waxman Foundations (W.A.W.), and the Burroughs Wellcome and Thrasher Research Funds (W.A.W.).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weiss, W., Taylor, S. & Shokat, K. Recognizing and exploiting differences between RNAi and small-molecule inhibitors. Nat Chem Biol 3, 739–744 (2007). https://doi.org/10.1038/nchembio1207-739
Issue Date:
DOI: https://doi.org/10.1038/nchembio1207-739
This article is cited by
-
Lysosomal control of senescence and inflammation through cholesterol partitioning
Nature Metabolism (2023)
-
Phosphodiesterase inhibitors in psychiatric disorders
Psychopharmacology (2023)
-
NADPH oxidase 4 contributes to TRPV4-mediated endothelium-dependent vasodilation in human arterioles by regulating protein phosphorylation of TRPV4 channels
Basic Research in Cardiology (2022)
-
Suppression of monosodium urate crystal-induced inflammation by inhibiting TGF-β-activated kinase 1-dependent signaling: role of the ubiquitin proteasome system
Cellular & Molecular Immunology (2021)
-
Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours
BMC Cancer (2020)