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Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over

Nature Genetics volume 13, pages 336–342 (1996)Cite this article

Abstract

Mice that are deficient in either the Pms2 or Msh2 DNA mismatch repair genes have microsatellite instability and a predisposition to tumours. Interestingly, Pms2–deficient males display sterility associated with abnormal chromosome pairing in meiosis. Here mice deficient in another mismatch repair gene, Mlh1, possess not only microsatellite instability but are also infertile (both males and females). Mlh 1 –deficient spermatocytes exhibit high levels of prematurely separated chromosomes and arrest in first division meiosis. We also show that Mlh1 appears to localize to sites of crossing over on meiotic chromosomes/Together these findings suggest that Mlh1 is involved in DNA mismatch repair and meiotic crossing over.

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Author notes

  1. Sean M. Baker and Annemieke W. Plug: S.M.B. and A.W.P. contributed equally to this study

Authors and Affiliations

  1. Department of Molecular and Medical Genetics, L103, Oregon Health Sciences University, 3181S. W. Sam Jackson Park Road, Portland, Oregon, 97201-3098, USA

    Sean M. Baker, C. Eric Bronner, Allie C. Harris, Donna-Marie Christie & R. Michael Liskay

  2. Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510, USA

    Annemieke W. Plug & Terry Ashley

  3. Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, 77030, USA

    Tomas A. Prolla & Allan Bradley

  4. Molecular Biology Program, University of Southern California, Los Angeles, California, 90089-1340, USA

    Xiang Yao & Norm Arnheim

  5. PharMingen, 10975 Torreyana Rd., San Diego, California, 92121, USA

    Craig Monell

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Correspondence to R. Michael Liskay.

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Baker, S., Plug, A., Prolla, T. et al. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet 13, 336–342 (1996). https://doi.org/10.1038/ng0796-336

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