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Assembly of microarrays for genome-wide measurement of DNA copy number

Nature Genetics volume 29, pages 263–264 (2001)Cite this article

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05–0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

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Figure 1: Measurement of single-copy changes.
Figure 2: Genome-wide copy-number variation in two breast tumors.

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Acknowledgements

We thank J. Flint, D. Ledbetter, C. Lese and Vysis, Inc. for telomere clones and clones containing certain named genes. This work was supported by NIH grants CA80314, CA83040, CA84118, HD17665 and CA58207, by California BCRP grant 2RB-0225 and by Vysis, Inc.

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Authors and Affiliations

  1. Comprehensive Cancer Center, University of California San Francisco, San Francisco, 94143, California

    Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Nils Brown, Greg Hamilton, Anna Katherine Hindle, Bing Huey, Karen Kimura, Sindy Law, Ken Myambo, Joel Palmer, Bauke Ylstra, Jingzhu Pearl Yue, Joe W. Gray, Ajay N. Jain, Daniel Pinkel & Donna G. Albertson

  2. Cancer Research Institute, University of California San Francisco, San Francisco, 94143, California

    Antoine M. Snijders, Stephanie Blackwood, Anna Katherine Hindle, Sindy Law, Joel Palmer, Bauke Ylstra, Ajay N. Jain & Donna G. Albertson

  3. Department of Laboratory Medicine, University of California San Francisco, San Francisco, 94143, California

    Joe W. Gray, Ajay N. Jain, Daniel Pinkel & Donna G. Albertson

  4. Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, 14263, New York

    Norma Nowak & Jeffrey Conroy

Authors
  1. Antoine M. Snijders
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  2. Norma Nowak
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  7. Greg Hamilton
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  8. Anna Katherine Hindle
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  9. Bing Huey
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  10. Karen Kimura
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  11. Sindy Law
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  12. Ken Myambo
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  13. Joel Palmer
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  15. Jingzhu Pearl Yue
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  16. Joe W. Gray
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  18. Daniel Pinkel
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  19. Donna G. Albertson
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Corresponding author

Correspondence to Donna G. Albertson.

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Snijders, A., Nowak, N., Segraves, R. et al. Assembly of microarrays for genome-wide measurement of DNA copy number. Nat Genet 29, 263–264 (2001). https://doi.org/10.1038/ng754

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