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Precis on forward genetics in mice

Nature Immunology volume 8, pages 659–664 (2007)Cite this article

Mutations have been the driving force behind some of the most important discoveries in immunology, and the growing speed with which they can be found has impelled the use of random mutagenesis to create new immunological phenotypes in mice. Here we describe how phenotypes are created, detected and ascribed to genetic change.

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Figure 1: Inbreeding protocols for generating homozygous mutations.
Figure 2: Steps in positional cloning of a recessive phenotype.
Figure 3: Integrated system for mutation identification.

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Acknowledgements

Supported by the National Institutes of Health (P01 AI070167).

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Authors and Affiliations

  1. Xin Du and Yu Xia are in the Department of Genetics, Bruce Beutler, The Scripps Research Institute, La Jolla, California 92037, USA. bruce@scripps.edu,

    Bruce Beutler, Xin Du & Yu Xia

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  1. Bruce Beutler
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Beutler, B., Du, X. & Xia, Y. Precis on forward genetics in mice. Nat Immunol 8, 659–664 (2007). https://doi.org/10.1038/ni0707-659

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