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References
Carlsson, R. & Glad, C. Monoclonal antibodies in the 90's: The all-purpose tool. Bio/Technology 7, 567–573 (1989).
Underwood, P.A. & Bean, P.A. Hazards of the limiting dilution method of cloning hybridomas. J. Immunol. Methods 107, 119–128 (1988).
Ozturk, S.S. & Palsson, B.A. Loss of antibody productivity during long term cultivation of a hybridoma cell line in low serum. Hybridoma 9, 167–175 (1990).
Gray, F., Kenney, J.S. & Dunne, J.F. Secretion capture and report web: Use of affinity-derivatized agarose microdrops for selection of hybridoma cells. J. Immunol. Methods 182, 155–163 (1995).
Kenney, J.S., Gray, F., Ancel, M.-H. & Dunne, J.F. Production of monoclonal antibodies using a secretion capture and report web. Bio/Technology 13, 787–790 (1995).
Weaver, J.C., Bliss, J.G., Harrison, G.I., Powell, K. & Williams, G.B. Microdrop technology: A method for separating cells by function and composition. Methods Enzymol. 2, 234–247 (1990).
Powell, K.T. & Weaver, J.C. Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells in a population. Bio/Technology 8, 333–337 (1990).
Weaver, J.C., Dunne, J.F., Gray, F., Lazzari, K.G. & Lin, J.B. Antibody secretion assay using gel microdrops and flow cytometry. in Flow Cytometry Applications in Animal Cell Culture. (eds. Al-Rubeai, M. & Emery, A.M.) 39–62 (Marcel Dekker, New York, 1995).
Weaver, J.C., Williams, G.B., Klibanov, A. & Demain, A.L. Gel microdrops: Rapid detection and enumeration of individual microorganisms by their metabolic activity. Bio/Technology 6, 1084–1089 (1988).
Williams, G.B., Weaver, J.C. & Demain, A.L. Rapid microbial detection and enumeration using gel microdroplets: Colorimetric versus fluorescent indicator systems. J. Clin. Microbiol. 28, 1002–1008 (1990).
Ryan, C., Nguyen, B.T. & Sullivan, S.J. A rapid assay for mycobacterial growth and antibiotic sensitivity using gel microdrop encapsulation. J. Clin. Microbiol. 33, 1720–1726 (1995).
Gift, E.A., Park, H., Paradis, G.A., Demain, Al. & Weaver, J.C. FACS-based isolation of slow-growing microorganisms: A model system involving double encapsulation of yeast in gel microdrops. Nature Biotechnol. 14, 884–887 (1995).
Nguyen, B.-T. et al. In situ hybridization to chromosomes stabilized in gel microdrops. Cytometry 21, 111–119 (1995).
Sullivan, S. & McGrath, P. Screening transfected cells for recombinant protein secretion using encapsulation and FACS. Mol. Biol. Cell 6, 445A (1995).
Uzgiris, A.J., Cuilfoyle, R.A. & Smith, L.M. Flow cytometric sorting of E. coli applied to cloning. in Genome Science and Technology (ed. Venter, J.C.) (Mary Ann Liebert, New York, 1994).
Parks, D.R., Bryant, V.M., Oi, V.T. St Herzenberg, L.A. Antigen specific identification and cloning of hybridomas with FACS. Proc. Natl. Acad. Sci. USA 76, 1962–1966 (1979).
Meilhoc, E., Wittrup, K.D. & Bailey, I.E. Application of flow cytometric measurements of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells. J. Immunol. Methods 121, 167–174 (1989).
Sen, S., Hu, W.S. & Srienc, F. Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production. Enzyme Micmb. Technol. 12, 571–576 (1990).
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Weaver, J., McGrath, P. & Adams, S. Gel microdrop technology for rapid isolation of rare and high producer cells. Nat Med 3, 583–585 (1997). https://doi.org/10.1038/nm0597-583
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DOI: https://doi.org/10.1038/nm0597-583
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