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Gel microdrop technology for rapid isolation of rare and high producer cells

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References

  1. Carlsson, R. & Glad, C. Monoclonal antibodies in the 90's: The all-purpose tool. Bio/Technology 7, 567–573 (1989).

    CAS  Google Scholar 

  2. Underwood, P.A. & Bean, P.A. Hazards of the limiting dilution method of cloning hybridomas. J. Immunol. Methods 107, 119–128 (1988).

    Article  CAS  Google Scholar 

  3. Ozturk, S.S. & Palsson, B.A. Loss of antibody productivity during long term cultivation of a hybridoma cell line in low serum. Hybridoma 9, 167–175 (1990).

    Article  CAS  Google Scholar 

  4. Gray, F., Kenney, J.S. & Dunne, J.F. Secretion capture and report web: Use of affinity-derivatized agarose microdrops for selection of hybridoma cells. J. Immunol. Methods 182, 155–163 (1995).

    Article  CAS  Google Scholar 

  5. Kenney, J.S., Gray, F., Ancel, M.-H. & Dunne, J.F. Production of monoclonal antibodies using a secretion capture and report web. Bio/Technology 13, 787–790 (1995).

    Article  CAS  Google Scholar 

  6. Weaver, J.C., Bliss, J.G., Harrison, G.I., Powell, K. & Williams, G.B. Microdrop technology: A method for separating cells by function and composition. Methods Enzymol. 2, 234–247 (1990).

    Article  Google Scholar 

  7. Powell, K.T. & Weaver, J.C. Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells in a population. Bio/Technology 8, 333–337 (1990).

    CAS  PubMed  Google Scholar 

  8. Weaver, J.C., Dunne, J.F., Gray, F., Lazzari, K.G. & Lin, J.B. Antibody secretion assay using gel microdrops and flow cytometry. in Flow Cytometry Applications in Animal Cell Culture. (eds. Al-Rubeai, M. & Emery, A.M.) 39–62 (Marcel Dekker, New York, 1995).

    Google Scholar 

  9. Weaver, J.C., Williams, G.B., Klibanov, A. & Demain, A.L. Gel microdrops: Rapid detection and enumeration of individual microorganisms by their metabolic activity. Bio/Technology 6, 1084–1089 (1988).

    CAS  Google Scholar 

  10. Williams, G.B., Weaver, J.C. & Demain, A.L. Rapid microbial detection and enumeration using gel microdroplets: Colorimetric versus fluorescent indicator systems. J. Clin. Microbiol. 28, 1002–1008 (1990).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Ryan, C., Nguyen, B.T. & Sullivan, S.J. A rapid assay for mycobacterial growth and antibiotic sensitivity using gel microdrop encapsulation. J. Clin. Microbiol. 33, 1720–1726 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Gift, E.A., Park, H., Paradis, G.A., Demain, Al. & Weaver, J.C. FACS-based isolation of slow-growing microorganisms: A model system involving double encapsulation of yeast in gel microdrops. Nature Biotechnol. 14, 884–887 (1995).

    Article  Google Scholar 

  13. Nguyen, B.-T. et al. In situ hybridization to chromosomes stabilized in gel microdrops. Cytometry 21, 111–119 (1995).

    Article  CAS  Google Scholar 

  14. Sullivan, S. & McGrath, P. Screening transfected cells for recombinant protein secretion using encapsulation and FACS. Mol. Biol. Cell 6, 445A (1995).

    Google Scholar 

  15. Uzgiris, A.J., Cuilfoyle, R.A. & Smith, L.M. Flow cytometric sorting of E. coli applied to cloning. in Genome Science and Technology (ed. Venter, J.C.) (Mary Ann Liebert, New York, 1994).

    Google Scholar 

  16. Parks, D.R., Bryant, V.M., Oi, V.T. St Herzenberg, L.A. Antigen specific identification and cloning of hybridomas with FACS. Proc. Natl. Acad. Sci. USA 76, 1962–1966 (1979).

    Article  CAS  Google Scholar 

  17. Meilhoc, E., Wittrup, K.D. & Bailey, I.E. Application of flow cytometric measurements of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells. J. Immunol. Methods 121, 167–174 (1989).

    Article  CAS  Google Scholar 

  18. Sen, S., Hu, W.S. & Srienc, F. Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production. Enzyme Micmb. Technol. 12, 571–576 (1990).

    Article  CAS  Google Scholar 

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Weaver, J., McGrath, P. & Adams, S. Gel microdrop technology for rapid isolation of rare and high producer cells. Nat Med 3, 583–585 (1997). https://doi.org/10.1038/nm0597-583

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