Abstract
Delivery of antigen in a manner that induces effective, antigen–specific immunity is a critical challenge in vaccine design. Optimal antigen presentation is mediated by professional antigen–presenting cells (APCs) capable of taking up, processing and presenting antigen to T cells in the context of costimulatory signals required for T–cell activation. Developing immunization strategies to optimize antigen presentation by dendritic cells, the most potent APCs, is a rational approach to vaccine design. Here we show that cutaneous genetic immunization with naked DNA results in potent, antigen–specific, cytotoxic T lymphocyte–mediated protective tumor immunity. This method of immunization results in the transfection of skin–derived dendritic cells, which localize in the draining lymph nodes. These observations provide a basis for further development of DNA–based vaccines and demonstrate the feasibility of genetically engineering dendritic cells in vivo.
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Condon, C., Watkins, S., Celluzzi, C. et al. DNA–based immunization by in vivo transfection of dendritic cells. Nat Med 2, 1122–1128 (1996). https://doi.org/10.1038/nm1096-1122
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DOI: https://doi.org/10.1038/nm1096-1122
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