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Transposon-tagged mutagenesis in the rat

Nature Methods volume 4, pages 131–133 (2007)Cite this article

Abstract

Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.

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Figure 1: Gene trapping by the SB system in the rat.
Figure 2: Successful insertional mutagenesis by the SB transposon system in the rat.
Figure 3: Successful generation and analyses of SB transposon insertional mutants in the rat.

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Acknowledgements

We thank P. B. Hackett (University of Minnesota) for providing SB cassettes.

Author information

Authors and Affiliations

  1. Division of Genome Dynamics, Laboratory of Mammalian Genetics, Creative Research Initiative “Sousei”, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060-0810, Japan

    Kazuhiro Kitada, Satoshi Ishishita & Keiko Tosaka

  2. Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060-0810, Japan

    Satoshi Ishishita

  3. The YS Institute, Inc., 1198-4, Iwazo, Utsunomiya, Tochigi, 321-0973, Japan

    Ri-ichi Takahashi & Masatsugu Ueda

  4. Center for Advanced Science and Innovation, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Vincent W Keng & Junji Takeda

  5. Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Kyoji Horie & Junji Takeda

Authors
  1. Kazuhiro Kitada
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  2. Satoshi Ishishita
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  3. Keiko Tosaka
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  4. Ri-ichi Takahashi
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  5. Masatsugu Ueda
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  6. Vincent W Keng
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  7. Kyoji Horie
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  8. Junji Takeda
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Corresponding author

Correspondence to Kazuhiro Kitada.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Lethality of Stch homozygous mutants revealed by testcross analysis.

Supplementary Table 1

Summary of the trapped genes in this study.

Supplementary Table 2

Summary of additional transposition sites.

Supplementary Methods

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Kitada, K., Ishishita, S., Tosaka, K. et al. Transposon-tagged mutagenesis in the rat. Nat Methods 4, 131–133 (2007). https://doi.org/10.1038/nmeth1002

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