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Fiber-optic fluorescence imaging

Nature Methods volume 2, pages 941–950 (2005)Cite this article

Abstract

Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.

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Figure 1: Photographs of photonic crystal fibers.
Figure 2: Scanning mechanisms.
Figure 3: An MEMS scanner.
Figure 4: Fiber-optic fluorescence imaging embodiments.

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Acknowledgements

Our work on fiber-optic imaging is supported by grants to M.J.S from the Human Frontier Science Program, the US National Institute on Drug Abuse, the US National Institute of Neurological Disorders and Stroke, the US National Science Foundation, the US Office of Naval Research, the Arnold & Mabel Beckman Foundation and the David & Lucille Packard Foundation. B.A.F is a National Science Foundation Graduate Research Fellow. E.D.C. is a member of the Stanford Biotechnology Program. W.P. is an affiliate of the National Electronics and Computer Technology Center of Thailand. E.L.M.C. is supported in part by a Dean's Fellowship, Stanford School of Medicine. We thank R. Kristiansen of C. Fibre for providing images of photonic crystal fiber.

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  1. James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, 318 Campus Drive, Stanford, 94305, California, USA

    Benjamin A Flusberg, Eric D Cocker, Wibool Piyawattanametha, Juergen C Jung, Eunice L M Cheung & Mark J Schnitzer

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  1. Benjamin A Flusberg
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Flusberg, B., Cocker, E., Piyawattanametha, W. et al. Fiber-optic fluorescence imaging. Nat Methods 2, 941–950 (2005). https://doi.org/10.1038/nmeth820

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