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An engineered ribonuclease preferring phosphorothioate RNA

Nature Structural Biology volume 5, pages 365–368 (1998)Cite this article

Abstract

We used mutants of RNase T1 and the Rp isomer of a thiosubstituted substrate to determine stereospecific thioeffects on catalysis. The analysis reveals subtle structural and functional changes in the Lntermolecular transition state interactions. Tyr 38 contributes to catalysis through a hydrogen bond with the pro-Rp oxygen. Y38F RNase T1 prefers the Rp thiosubstituted analog over the natural phosphodiester substrate that is favored by the wild type enzyme.

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Authors and Affiliations

  1. Dienst Ultrastructuur, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640, Sint-Genesius-Rode, Belgium

    Stefan Loverix & Jan Steyaert

  2. Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-10691, Stockholm, Sweden

    Anna Winquist

  3. Division of Organic and Bioorganic Chemistry, MBB, Scheele Laboratory, Karolinska Institutet, S-17177, Stockholm, Sweden

    Roger Strömberg

Authors
  1. Stefan Loverix
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  2. Anna Winquist
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  3. Roger Strömberg
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  4. Jan Steyaert
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Correspondence to Jan Steyaert.

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Loverix, S., Winquist, A., Strömberg, R. et al. An engineered ribonuclease preferring phosphorothioate RNA. Nat Struct Mol Biol 5, 365–368 (1998). https://doi.org/10.1038/nsb0598-365

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