Abstract
To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.
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Acknowledgements
This work was supported by grants from the US National Institutes of Health (AI44626) and the US National Science Foundation (CHE-99-09502), Florida State University and the US National High Magnetic Field Laboratory.
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Supplementary information
Supplementary Fig. 1
SDS-PAGE of immature and mature VLPs. (PDF 71 kb)
Supplementary Fig. 2
Exchange profiles for CA peptides. (PDF 135 kb)
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Lanman, J., Lam, T., Emmett, M. et al. Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange. Nat Struct Mol Biol 11, 676–677 (2004). https://doi.org/10.1038/nsmb790
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DOI: https://doi.org/10.1038/nsmb790
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