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Autografting

Impaired bone marrow hematopoietic progenitor cell function in rheumatoid arthritis patients candidated to autologous hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 33, pages 721–728 (2004)Cite this article

Summary:

We have evaluated bone marrow morphology, percentage of bone marrow CD34+ cells, proliferative activity of bone marrow precursors, clonogenic assay (BFU-E and CFU-GM) in short-term bone marrow cultures, and bone marrow cell apoptosis, together with serum TNF-α and IL-6, in 16 chronic, refractory RA patients, as well as in five healthy controls. Of 16 RA patients (68.7%), 11 showed a reduced bone marrow cellularity, while it was normal in all the controls. In RA patients, the median percentage of CD34+ bone marrow cells, the median percentage of proliferating bone marrow myeloid precursors, and the median number of both BFU-E and CFU-GM colonies were significantly lower than observed in the controls. As far as TNF-α and IL-6 titers is concerned, the latter did not significantly differ from controls' values, while TNF-α titers were significantly lower in healthy controls. Finally, the median apoptotic index of early bone marrow myeloid cells of RA patients was significantly higher compared with controls. These observations may identify the biological risk factors for impaired mobilization and/or engraftment when RA patients are candidates for autologous hematopoietic stem cell grafting.

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Authors and Affiliations

  1. Istituto di Medicina Interna ed Oncologia Medica, Università degli Studi di Pavia e I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

    C Porta, I Ramaioli, R Invernizzi, B Rovati & M Danova

  2. Laboratorio di Citometria e Terapie Cellulari, Università degli Studi di Pavia e I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

    C Porta, B Rovati & M Danova

  3. Servizio di Reumatologia, Università degli Studi di Pavia e I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

    R Caporali, O Epis & C Montecucco

  4. Laboratori Area Trapiantologica e Biotecnologica, Università degli Studi di Pavia e I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

    I Ramaioli & G Comolli

  5. Servizio di Virologia, Università degli Studi di Pavia e I.R.C.C.S. Policlinico San Matteo, Pavia, Italy

    G Comolli

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  1. C Porta
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Correspondence to C Porta.

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Porta, C., Caporali, R., Epis, O. et al. Impaired bone marrow hematopoietic progenitor cell function in rheumatoid arthritis patients candidated to autologous hematopoietic stem cell transplantation. Bone Marrow Transplant 33, 721–728 (2004). https://doi.org/10.1038/sj.bmt.1704407

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