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Cloning of murine IL-22 receptor alpha 2 and comparison with its human counterpart

Genes & Immunity volume 5, pages 330–336 (2004)Cite this article

Abstract

We have identified the mouse and rat homologs of human interleukin-22 receptor alpha 2 (IL-22Rα2) and compared the localization, structure, and expression of the encoding murine and human genes. The mouse IL-22Rα2-encoding gene is located on chromosome 10A3 between, like in human, the genes for interferon-gamma R1 and IL-20R1. It spans a region of approximately 10 kb therefore being three times shorter than the human gene. Although the overall gene structure in both species is similar, the mouse gene lacks a counterpart to the third coding exon of the human gene known to be alternatively spliced. Like in human, mouse and rat IL-22Rα2 exist only as soluble receptors as deduced from the lack of transmembrane and intracellular domains encoding sequences. Quantitative expression analyses showed, analogically to the human system, a limited tissue distribution of mouse IL-22Rα2 mRNA. Differential modulation of IL-22Rα2 mRNA expression was observed upon systemic inflammation in mice in spleen, thymus, and lymph node.

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Acknowledgements

We thank A Buss, M Nieter, C Opitz, E Matzke and A Häussler-Quade for technical support. We also thank Dr AC Looman for gel electrophoresis analysis of murine IL-22Rα2. We are also very grateful to P Zambon for proofreading the manuscript.

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Author notes

  1. B Weiss and K Wolk: These authors contributed equally to this study.

Authors and Affiliations

  1. Enabling Technologies, Schering AG, Berlin, Germany

    B Weiss

  2. CRBA Dermatology, Schering AG, Berlin, Germany

    K Wolk, B H Grünberg, K Asadullah & R Sabat

  3. Interdisciplinary Group of Molecular Immunopathology, Dermatology / Medical Immunology, University Hospital Charité, Berlin, Germany

    K Wolk & R Sabat

  4. Institute of Medical Immunology, University Hospital Charité, Berlin, Germany

    H-D Volk

  5. Department of Dermatology, Venerology and Allergology, University Hospital Charité, Berlin, Germany

    W Sterry

Authors
  1. B Weiss
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  2. K Wolk
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  3. B H Grünberg
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  4. H-D Volk
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  5. W Sterry
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  6. K Asadullah
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  7. R Sabat
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Corresponding author

Correspondence to R Sabat.

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Weiss, B., Wolk, K., Grünberg, B. et al. Cloning of murine IL-22 receptor alpha 2 and comparison with its human counterpart. Genes Immun 5, 330–336 (2004). https://doi.org/10.1038/sj.gene.6364104

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  • DOI: https://doi.org/10.1038/sj.gene.6364104

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