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Differential regulation of human RecQ family helicases in cell transformation and cell cycle

Oncogene volume 19, pages 4764–4772 (2000)Cite this article

Abstract

Three human RecQ DNA helicases, WRN, BLM and RTS, are involved in the genetic disorders associated with genomic instability and a high incidence of cancer. RecQL1 and RecQL5 also belong to the human RecQ helicase family, but their correlation with genetic disorders, if any, is unknown. We report here that in human B cells transformed by Epstein-Barr virus (EBV), human fibroblasts and umbilical endothelial cells transformed by simian virus 40, the expression of WRN, BLM, RTS and RecQL1 was sharply up-regulated. In B cells this expression was stimulated within 5–40 h by the tumor promoting agent phorbol myristic acetate (PMA). Interestingly, RecQL5β, an alternative splicing product of RecQL5 with a nuclear localization signal, is expressed in resting B cells without significant modulation of its synthesis by EBV or PMA, suggesting it has a role in resting cells. We also roughly determined the number of copies per cell for the five RecQ helicase in B cells. In addition, levels of the different RecQ helicases are modulated in different ways during the cell cycle of actively proliferating fibroblasts and umbilical endothelial cells. Our results support the view that the levels of WRN, BLM, RTS and RecQL1 are differentially up-regulated to guarantee genomic stability in cells that are transformed or actively proliferating.

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Authors and Affiliations

  1. AGENE Research Institute, 200 Kajiwara, Kamakura, Kanagawa, 247-0063, Japan

    Tamae Kawabe, Saori Kitao, Kaori Nishikawa, Akira Shimamoto, Miwa Shiratori, Takehisa Matsumoto, Yasuhiro Furuichi & Masanobu Sugimoto

  2. Department of Cellular and Molecular Biology, Hiroshima University School of Medicine, Minami-ku, Hiroshima, 734-8551, Japan

    Naohiro Tsuyama, Kumiko Anno, Tatsuhiro Sato & Toshinori Ide

  3. National Institute of Bioscience & Human-Technology Higashi 1-1, Tsukuba, Ibaraki, 305-8566, Japan

    Youji Mitsui

  4. Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980-8578, Miyagi, Japan

    Masayuki Seki & Takemi Enomoto

  5. Department of Rheumatology, Tokyo Metropolitan Otsuka Hospital, Minami-Otsuka, Toshima-ku, Tokyo, 170-0005, Japan

    Makoto Goto

  6. Department of Human Genetics, Laboratory of Cancer Susceptibility, Memorial Sloan-Kettering Cancer Center, New York, 10021, NY, USA

    Nathan A Ellis

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  1. Tamae Kawabe
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Kawabe, T., Tsuyama, N., Kitao, S. et al. Differential regulation of human RecQ family helicases in cell transformation and cell cycle. Oncogene 19, 4764–4772 (2000). https://doi.org/10.1038/sj.onc.1203841

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